气相色谱串接质谱快速检测不同类别食品中的邻苯二甲酸酯

Fast Determination of Phthalate Esters in Different Types of Food by Gas Chromatography Tandem Mass Spectrometry

目的建立食品中16种塑化剂的快速检测方法。方法采用简化净化步骤,不同试样采用不同的萃取溶剂,然后用固相萃取柱净化、洗脱,洗脱液浓缩后,用气相色谱-串接质谱仪测定,内标法定量测定不同食品中16种邻苯二甲酸酯。结果 16种邻苯二甲酸酯的线性关系较好,方法检测限范围为0.01~0.03 mg/kg。在高中低3个水平下进行加标实验,得到的平均回收率为65.7%~115.3%,相对标准偏差小于15%（n=6）。结论该方法简便快速,溶剂消耗量小,且应用串接质谱更好地消除了基质效应的干扰,灵敏度高,定性更准确。

To develop a fast and accurate method with simple pretreatment and good selectivity to detect 16 plasticizers in different types of foods in this study. After extraction of different types of foods using different solvents,the phthalate esters（PAEs） were further cleaned up and eluted by solid phase extraction. Then after concentration, the extracts were directly injected into gas chromatography tandem mass spectrometry（GC-MS/MS）. The 16 PAEs in different types of foods were determined using the internal standard method. There was a good linear relation among the 16 PAEs, and the method quantification limits were between 0.01 mg/kg and 0.03 mg/kg. Spiking experiments at high, medium and low levels revealed that the average recoveries ranged from 65.7% to 115.3%, and the relative standard deviations were lower than 15%（n=6）. The method was shown to be rapid and reliable, with low solvent consumption. The application of tandem mass spectrometry better eliminated the interference of matrix effects, resulting in high sensitivity and more accurate qualitative measurements.