结核分枝杆菌特异性抗原检测在临床诊断中的意义

Significance of Mycobacterium Tuberculosis-Specific Antigens Detection in the Clinical Diagnosis

目的探讨结核菌特异性抗原ESAT-6及CFP-10在肺结核及肺炎患者中诊断与鉴别诊断的意义。方法就我院肺炎患者20例,肺结核患者40例,通过ELISA实验及PPD实验,对2组人群进行比较,研究结核菌特异性抗原在肺结核及肺炎患者中诊断与鉴别诊断的意义。结果通过ELISA法对二组人群进行结核菌检测的阳性率的比较,肺结核组与肺炎组相比,P<0.01。通过皮试法(TST法)进行结核菌素实验对二组人群结核菌阳性率的比较,肺结核组与肺炎组相比,P>0.05,无统计学意义。结论 ELISA实验法在两组人群的对照中灵敏度特异度均普遍高于PPD实验方法,并且在肺结核和肺炎的鉴别诊断中有统计学意义,说明该实验在临床中对肺结核的鉴别诊断是有价值的。

Objective To explore the clinical significance of mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 in the diagnosis and identification of tuberculosis and pneumonia. Methods A total of 20 pneumonia patients and 40 tuberculosis patients were selected, and compared between these two groups through ELISA and PPD methods, in order to study the clinical significance of mycobacterium tuberculosis-specific antigens in the diagnosis and identification of tuberculosis and pneumonia. Results The comparison of the positive rate of mycobacterium tuberculosis detection with ELISA method showed that P〈0.01 in the tuberculosis group and pneumonia group. PPD method was used to conduct the tuberculin test, and the comparison of the positive rate between two groups showed there was no significant dif erence between tuberculosis group and pneumonia group （P〉0.05）. Conclusion ELISA method has the higher sensitivity and specificity than PPD method in comparison between two grups, and has statistical significance in the diagnosis and identification of tuberculosis and pneumonia, indicating that it has the significance in the clinical diagnosis and identification of tuberculosis.