GSTP1基因多态性与新疆维、汉肺癌易感性的关系

Relationship between GSTP1 genetic polymorphism and susceptibility of lung cancer in Xinjiang Uygurs and Hans

目的探讨谷胱甘肽S转移酶P1基因（glutathione S-transferase P1,GSTP1）rs1695位点多态性与新疆地区维吾尔族（维族）和汉族肺癌患者的相关性。方法采用病例—对照研究方法,选取维族、汉族肺癌患者各80例作为病例组,另以维族、汉族健康人群各80例为对照组,运用限制性片段长度多态性聚合酶链反应（PCRrestriction fragment length polymorphism,PCR-RFLP）技术检测GSTP1基因Ile105Val多态性,分析其基因型频率在2个民族间分布的差异。结果 （1）GSTP1基因rs1695位点多态性在对照组与病例组中的分布均符合HardyWeinberg平衡;（2）在维族人群中,GSTP1基因rs1695位点基因型在病例组与对照组中的分布频率差异无统计学意义（P〉0.05）。在汉族人群中,携带G等位基因者发生肺癌的风险增加（OR=2.170,95%CI：1.146~4.107,P〈0.05）;（3）维族人群突变型杂合子AG和纯合子GG基因型频率均高于汉族,其中在对照组中维族GSTP1（GG）基因型频率较汉族高1.3倍（8.8%vs 3.8%）,但差异均无统计学意义（均P〉0.05）。结论 GSTP1基因rs1695多态性与汉族人群肺癌发病风险相关,与维吾尔族人群无关,其相关性具有民族差异。

Objective To investigate the association between glutathione S-transferase P1（GSTP1） genetic polymorphism and lung cancer susceptibility in Xinjiang Uygurs and Hans.Methods PCR-restriction fragment length polymorphism（PCR-RFLP） analysis was used to detect the polymorphism of GSTP1 gene in 80 Uygur patients and 80 Han patients（case group）,and 80 healthy Uygur subjects and 80 healthy Han subjects（control group）.Difference in genotype frequencies between ethnic Uygurs and Hans was also analyzed.Results The genotype frequencies of GSTP1 gene rs1695 variants were in Hardy-Weinberg equilibrium in both case and control groups.In Uygur population,the genotype frequencies of GSTP1 at rs1695 site showed no significant difference between case and control groups（P 〉 0.05）.In Han population,there was significant difference between two groups（P 〈 0.05）.Han individuals with G allele had increased risk of developing lung cancer（OR = 2.170,95% CI：1.146 ~ 4.107,P 〈 0.05）.In both case and control groups,AG and GG genotype frequencies in Uygurs were higher than those in Hans.For control group,GG genotype frequency in Uygurs was1.3 times higher than that in Hans（8.8% vs 3.8%,P 〉 0.05）.Conclusion The genetic polymorphism of GSTP1 is associated with susceptibility to lung cancer in Han population,but not in Uygur population.