瘤内局部注射重组人血管内皮抑制素对小鼠Lewis肺癌抑瘤作用的研究

Recombinant human endostatin suppresses Lewis lung cancer in mice:intratumorous versus intraperitoneal injection

目的探讨瘤内直接注射重组人血管内皮抑制素(恩度)联合腹腔注射顺铂对Lewis肺癌的抑制效果。方法成功建立小鼠Lewis肺癌移植瘤模型。按体质量将小鼠随机分为生理盐水组、顺铂组、瘤内注射恩度组、顺铂联合腹腔注射恩度组、顺铂联合瘤内注射恩度组5组。治疗后处死小鼠,计算肿瘤体积抑瘤率,用免疫组织化学法测定各组小鼠肿瘤组织血管内皮生长因子(VEGF)的表达水平及微血管密度(microvessel density,MVD)。结果顺铂组、瘤内注射恩度组、顺铂联合腹腔注射恩度组、顺铂联合瘤内注射恩度组的抑瘤率分别为61.8%、24.9%、70.3%、74.3%。免疫组织化学结果显示,顺铂联合瘤内注射恩度组的VEGF表达及MVD计数在各实验组中均最低,与顺铂联合腹腔注射恩度组比较两者差异均有统计学意义(均P<0.05)。结论经皮穿刺瘤内注射恩度联合顺铂有协同抗肿瘤作用,能有效抑制肺癌的生长及转移,并且相同剂量的恩度,肿瘤内局部注射效果优于腹腔注射。

Objective To investigate the effect of intratumorous injection of recombinant human endostain（Endostar） combined with cisplatin on the growth of Lewis lung cancer in mice.Methods The Lewis lung cancer model was established in C57 BL/6 mice.Tumor-bearing mice were randomly divided into 5 groups：group A（control group）,group B（intraperitoneal injection of cisplatin）,group C（intratumorous injection of Endostar）,group D（intraperitoneal injection of cisplatin and Endostar）,group E（intraperitoneal injection of cisplatin and intratumorous injection of Endostar）.Tumor volume was measured on d11 and tumor inhibition rate（TIR） was calculated.The expression of VEGF and microvessel density（MVD） in tumor was detected by immunohistochemistry.Results Compared to group A,TIRs of groups B,C,D and E were 61.8%,24.9%,70.3% and 74.3%,respectively.The expression of VEGF and MVD of group E were the lowest among all groups,significantly lower than that of group D（both P 〈 0.05）.Conclusion Intratumorous injection of Endostar in combination with cisplatin exerts inhibitory effect on the growth of Lewis lung cancer in mice,and the effect was more marked than that of intraperitoneal injection.