二十二碳六烯酸下调心房颤动大鼠心房肌细胞双孔内向整流相关性钾通道-1的表达

The TREK-1 expression downregulation induced by docosahexaenoic acid in atrial myocytes of atrial fibrillation rats

目的:探讨二十二碳六烯酸(DHA)在大鼠心房颤动(房颤)模型中的作用及其对双孔内向整流相关性钾离子通道1(TREK-1)表达的影响。方法:将乙酰胆碱-氯化钙混合液敏感的雄性SD大鼠随机分为对照组(A组)、对照DHA处理组(B组)、房颤组(C组)、房颤DHA处理组(D组)。分别观察房颤发生时间和心房有效不应期(ERP);应用Western blot测定大鼠心房肌中TREK-1表达及反转录-聚合酶链反应测定大鼠心房肌中TREK-1 mRNA表达。结果:D组较C组大鼠房颤出现时间明显推迟,每天房颤持续时间显著缩短(P<0.01);与A组比较,C组大鼠心房ERP明显缩短,D组大鼠心房ERP较C组明显延长(P<0.01)。与A组比较,C组TREK-1 mRNA和蛋白表达水平均明显升高(P<0.01);与C组比较,D组TREK-1mRNA和蛋白表达均降低(P<0.05和P<0.01)。结论:DHA具有抗房颤作用,其机制可能与下调双孔钾通道TREK-1表达有关。

Objective：To explore the role of docosahexaenoic acid（DHA） in atrial fibrillation rat,and its effects on the expression of TWIK-Related K＋Channel 1（TREK-1）. Methods：Sprague-Dawley（SD） rats sensitive to acetylcholine-calcium chloride mixture were randomly divided into the control group（ group A）,DHA treatment control group（ group B）,atrial fibrillation（ group C） and DHA treatment atrial fibrillation group（ group D）. The duration of atrial fibrillation and atrial effective refractory period（ ERP） were observed. The expressions of TREK-1 protein and mRNA in atrial muscle of rats were detected using Western blot and reverse transcriptase-polymerase chain reaction. Results：Compared with group C,the occurrence and duration time of atrial fibrillation in group D were significantly delayed and shorten,respectively（ P〈0. 01）. Compared with group A and D,the atrial ERP in group C was significantly shorten（P〈0. 01）. Compared with group A and D,the expressions of TREK-1 mRNA and protein in group C increased significantly（P〈0. 01）. Conclusions：DHA may play the role of anti-atrial fibrillation,the mechanism of which may be related to the expression downregulation of TREK-1.