CD133在甲状腺乳头状癌中的表达及其与细胞外信号调节激酶1表达的关系

Expressions of CD133 and extracellular signal-regulated protein kinase 1 in papillary thyroid carcinoma and their correlations

目的：检测肿瘤干细胞标志物CD133在甲状腺乳头状癌（PTC）组织中表达及其与细胞外信号调节激酶1（ERK1）的关系及其临床病理意义。方法：采用免疫组织化学EliVisionTMplus法检测68例PTC组织和40例结节性甲状腺肿组织中CD133和ERK1的表达情况。结果：在结节性甲状腺肿组织和PTC组织中,CD133和ERK1的阳性率分别为10.0%与50.0%和12.5%与63.2%,2组差异均有统计学意义（P〈0.01）。CD133的表达水平在PTC组织的不同分化程度、淋巴结转移和临床分期间差异均有统计学意义（P〈0.05-P〈0.01）;PTC组织的分化越差,淋巴结发生转移及临床分期越高,ERK1的阳性率亦越高（P〈0.01）。CD133与ERK1的表达呈正相关关系（P〈0.05）。结论：CD133和ERK1的异常表达参与了PTC的发生、发展、浸润及转移;早期联合检查CD133和ERK1对PTC的进展及预后判断均有临床意义。

Objective：To detect the expressions of CD133,cancer stem cells marker,and extracellular signal-regulated protein kinase 1（ERK1） in papillary thyroid carcinoma（ PTC）,and their correlations and clinicopathological significance. Methods： The expressions of CD133 and ERK1 in 68 samples of PTC tissue and 40 samples of nodular goiter tissue were detected by immunohistochemistry. Results：The positive rates of CD133 and ERK1 in PTC and nodular goiter tissues were 10. 0% 50. 0% and12. 5% 63. 2%,respectively,the differences of which were statistically significant（ P〈0. 01）. The differences of the expression levels of CD133 in tumor grade,lymph node metastasis and TNM stage of PTC were statistically significant（ P〈0. 05 to P〈0. 01）.With the PTC worsening differentiation,lymph node transferring and clinical stage increasing,the positive rates of ERK1 increased（P〈0. 01）. There was a positive correlation between the expression of CD133 and ERK1（ P〈0. 05）. Conclusions： The abnormal expressions of CD133 and ERK1 may be involved in the occurrence,development,invasion and metastasis of PTC. The early combination examination of CD133 and ERK1 has a certain clinical significance in the progression and prognosis of PTC.