孕酮对自然流产小鼠白细胞介素6表达的影响

Effect of progesterone on the expression of IL-6 in spontaneous abortion mouse

目的：探讨孕酮对自然流产小鼠母胎界面白细胞介素6（IL-6）的调节作用.方法：建立经典的自然流产动物模型（CBA/J×DBA/2）,用免疫组织化学显色和RT-PCR检测自然流产小鼠蜕膜及绒毛组织中IL-6蛋白和mRNA的表达情况.结果：孕酮组血清孕酮水平明显高于模型组,IL-6水平高于模型组.孕酮组血清孕酮、IL-6水平与正常组比较均无差异.免疫组织化学显色和RT-PCR结果显示,在绒毛组织中,孕酮组IL-6蛋白相对含量显著高于模型组,IL-6 mRNA相对含量高于模型组,两者与正常组比较均无差异;在蜕膜组织中,孕酮组IL-6蛋白的相对含量低于模型组,IL-6 mRNA相对含量高于模型组,与正常组比较均无差异.结论：孕酮通过上调IL-6的表达来减少自然流产的发生和发展,而且孕酮调节Th1/Th2细胞因子平衡的作用发生在母胎界面.

Objective：To investigate the modulatory effect of progesterone on spontaneous abortion mouse fetal maternal interface IL-6.Methods：In the classical animal model of spontaneous abortion （CBA/J × DBA/2）,the expression of IL-6 protein and mRNA in spontaneous abortion mouse decidual and villi were measured by immunohistochemical staining method and RT-PCR.Results：In the progesterone group,the level of serum progesterone was significantly higher than that in the model group,and IL-6 level was higher than that in the model group.The level of IL-6 and progesterone in the progesterone group,showed no significant difference comparing with the normal group.Immunohistochemical staining and RT-PCR results showed：in the chorionic villi,the relative content of IL-6 protein in the progesterone group was significantly higher than that in the model group,the relative content of IL-6 mRNA was higher than that in the model group,and there was no significant difference compared with the normal group.In the decidual tissues,the relative content of IL-6 protein in the progesterone group was lower than that in the model group,IL-6 mRNA content was higher than that of the model group,and there was no significant difference compared with the normal group.Conclusion：The up-regulation of IL-6 expression of progesterone may reduce the occurrence and development of spontaneous abortion,and progesterone regulating Th1/Th2 cytokines balance occurs in the maternal fetal interface.