不同剂量乌司他丁对百草枯急性肺损伤大鼠肝肾功能的影响

Effects of Different Dose of Ulinastatin in Hepatic and Renal Function on Rats with Paraquat Induced Acute Lung Injury

目的:观察不同剂量乌司他丁(UTI)对百草枯(PQ)急性肺损伤大鼠肝肾功能的影响。方法:将50只SD大鼠随机分为正常对照组(A)、PQ中毒组(B)、小剂量UTI治疗组(C)、中剂量UTI治疗组(D)、大剂量UTI治疗组(E)五组,每组10只。B、C、D、E组大鼠采用PQ一次性灌胃染毒法(80 mg/kg)复制PQ中毒模型,A组采用等量生理盐水灌胃。灌胃30 min后,C组腹腔注射UTI 100 k U/kg,D组腹腔注射UTI 300 k U/kg,E组腹腔注射UTI 600 k U/kg,A、B组注射等量生理盐水。24 h后检测血清ALT、AST、BUN、Cr浓度,光镜下观察肺组织病理学变化,进行肺损伤评分。结果 :C、D、E组和B组比较,大鼠血清ALT、AST、BUN、Cr浓度及肺组织损伤病理评分存在显著差异(P<0.05);C、D、E组之间比较,血清ALT、AST、BUN、Cr浓度无显著差异(P>0.05),肺组织损伤病理评分存在显著差异(P<0.05)。结论:较大剂量UTI对急性PQ中毒大鼠急性肺损伤具有较强保护作用,对肝肾功能无明显影响。

Objective： To observe the effect of different dose of ulinastatin（UTI） in hepatic and renal function on rats with paraquat（PQ） induced acute lung injury. Methods： 50 SD rats were randomly divided into 5 groups, normal control group（A）, PQ poisoning group（B）, low dose UTI treatment group（C）, middle dose UTI treatment group（D）, high dose UTI treatment group（E）, 10 rats in each group. B,C, D, E group of rats were made into the poisoning model by intragastric PQ（80 mg/kg） administration, and same quantity of physiological saline was administered intragastrically in group A. 30 minutes after PQ administration, group C were intraperitoneally injected with UTI（100 kU/kg）, group D were intraperitoneally injected with UTI（300 kU/kg）, group E were intraperitoneally injected with UTI（600 kU/kg）, and then the A,B group were injected same quantity of physiological saline. The levels of serum ALT, AST, BUN,Cr concentration of rats after 24 hours were measured, the pathological changes of lung tissues, lung injury score under the light microscope were observed. Results： Compared with B group, there were significant differences in C, D, E group with the serum ALT,AST, BUN, Cr concentration and lung tissue pathological score（P〈0.05）; and serum ALT, AST, BUN, Cr concentration had no significant difference between C, D, E group（P〉0.05）, there were significant differences in pathological lung tissue injury score（P〉0.05）.Conclusion： High dose UTI has strong protective effect on acute lung injury induced by paraquat-intoxicated in rats without significant effect on liver and renal function.