细菌纤维素在模拟体液中的降解研究

Research on Bacterial Cellulose Degradation in a Simulated Body Fluid

研究了不同性质的纤维素酶对细菌纤维素(BC)的降解活性,着重探讨了在纤维素酶作用下,影响BC在模拟人体环境中降解行为的因素。研究表明中性纤维素酶在模拟人体环境的条件下对BC具有较高的降解活性。纤维素酶在模拟体液中的活性会随着时间延长而降低,但并未丧失活性,纤维素酶在模拟人体的环境下依然具有长期降解BC的作用。β-葡糖苷酶对BC的降解有很大的促进作用,当β-葡萄糖苷酶在复合酶中质量分数为60%时,复合酶降解BC膜的速率达到最大。

The degradation process of bacterial cellulose （BC） by different cellulase was studied,especially the factors that influence the degradation of BC in a simulated body fluid.The results showed that neutral cellulase had higher activity in a simulated body environment.Cellulase activity reduced along with time in a simulated body fluid but there was no deactivation.BC could be degraded by cellulase in the long term.At the same time,the β-glucoside enzyme played a great role in promoting BC degradation.When the ratio came to 60％,the rate of BC degradation was the highest.