摘要
为明确多菌灵质量浓度与蚯蚓体内乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GSTs)和羧酸酯酶(CarE)3种解毒酶活性之间的剂量效应关系,为多菌灵的安全使用及对相关动物的毒害研究提供参考,利用赤峰周边地区蔬菜大棚的蚯蚓,进行多菌灵0.7 5g/L、1.00g/L、1.25g/L、1.50g/L、1.75g/L和2.00g/L 6个质量浓度对蚯蚓体内3种解毒酶活性的影响试验。结果表明:与对照组(蒸馏水)相比,多菌灵对蚯蚓体内的乙酰胆碱酯酶整体起抑制作用,在多菌灵浓度为1.75 g/L时酶活性最低,为105.625μmol/L;对谷胱甘肽S-转移酶整体起诱导促进作用,在多菌灵浓度为1.00g/L时酶活性最高,为17.343μmol/L;对羧酸酯酶活性,在多菌灵浓度小于1.25g/L时表现为诱导促进作用,大于1.25g/L时表现为抑制作用。
The healthy earthworms from vegetable greenhouses in Chifeng City were treated with carbendazim with six mass concentrations（0.75g/L,1.00g/L,1.25g/L,1.50g/L,1.75g/L and 2.00g/L）to study the dose-effect relationship between carbendazim concentration and activity of AChE,GSTs and CarE and to provide a reference for safe use and poison research of carbendazim.Carbendazim has the inhibition effect on AChE activity overall and the AChE activity is 105.625 mol/L（minimum）when carbendazim concentration is 1.75g/L.Carbendazim has the promotion effect on GSTs activity overall and the GSTs activity is up to 17.343 mol/L（maximum）when carbendazim concentration is 1.00g/L.The CarE activity is induced and promoted when carbendazim concentration is less than 1.25g/L,and the CarE activity is inhibited when carbendazim concentration is higher than 1.25g/L.
出处
《贵州农业科学》
CAS
北大核心
2014年第12期177-179,共3页
Guizhou Agricultural Sciences
基金
内蒙古自治区教育厅高等学校研究项目"蚯蚓的生态毒理与环境安全指示研究"(NJ10249)
