东兰乌鸡神经肽Y基因对300日龄产蛋数的影响

Effects of NPY Gene on Total Number of Eggs with 300 Age in Donglan Black-bone Chicken

采用PCR-RFLP技术检测135只东兰乌鸡中NPY基因的多态性,并分析其对300日龄产蛋数的影响。结果表明,在该东兰乌鸡群体中发现了NPY基因的3种基因型(AA、AB、BB),不同基因型对300日龄产蛋数影响显著(P<0.05),AA型个体的产蛋数显著高于BB型个体(P<0.05)。因此,该NPY基因多态位点可以作为影响东兰乌鸡产蛋数的分子标记。

We used PCR-RFLP to test genetic polymorphism of NPY gene,and analyzed the effect of various genotypes on the total number of eggs with 300 age（ EN） in 135 Donglan black-bone chickens. The results showed that there were three genotypes in the group. The association analysis showed that NPY gene had significant effects on EN（ P〈0. 05）,and EN of AA genotype was higher than BB genotype（ P〈0.05）. So,the polymorphic site of NPY gene can be a good DNA marker to be used in MAS for EN in Donglan black-bone chicken.