精子DNA完整性与IVF/ICSI助孕结局的关系

Relationship between sperm DNA integrity and the outcome of in vitro fertilization/intracytoplasmic sperm injection

目的:探讨精子DNA碎片与体外受精/卵胞浆内单精子注射(IVF/ICSI)结局的关系。方法:采用精子染色质扩散实验(SCD)对接受IVF/ICSI治疗的280例(IVF 208例、ICSI 72例)男性精液样本进行DNA碎片指数(DFI)检测。根据检测结果将精液样本划分为低DFI(<25%)组和高DFI(≥25%)组,并比较两组的受精率、卵裂率、优胚率、可利用胚胎率及临床妊娠率。结果:无论选择何种受精方式,低DFI组和高DFI组的受精率、卵裂率、优胚率、胚胎利用率及临床妊娠率均无统计学差异(P>0.05)。结论:DFI的高低对IVF/ICSI的各种指标及助孕结局的影响不大。

Objective：To explore the relationship between sperm DNA fragmentation and the outcome of in vitro fertilization （IVF）/intracytoplasmic sperm injection （ICSI）.Methods：Sperm chromatin dispersion （SCD） was performed to determine DNA fragmentation index （DFI） in 208 IVF and 72 ICSI cycles.According to the test results,we divided the samples into low DFI group （〈25%） and high DFI group （≥25%）.The relationship between sperm DNA fragmentation and the fertilization rate,cleavage rate,good quality embryo rate,utilization rate of embryo and clinical pregnancy rate of two groups were compared.Results：No matter which fertilization method we chose,the fertilization rate,cleavage rate,good quality embryo rate,utilization rate of embryo and clinical pregnancy had no significant differences between the low DFI group and the high DFI group.Conclusion：DNA fragmentation rate may not affect the studied indicators and pregnancy outcome of IVF/ICSI.