三棱的环二肽类成分抗凝活性

Anticoagulant activity of cyclic dipeptides from Sparganii Rhizome

目的研究从三棱中分离得到的环-(酪氨酸-亮氨酸)、环-(苯丙氨酸-苯丙氨酸)和环-(苯丙氨酸-酪氨酸)这3个环二肽类成分的体外抗凝活性。方法使用试剂盒测定三棱乙醇提液分离得到3个环二肽类成分对取自雄性SD大鼠腹主动脉贫血小板血浆的凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)及凝血酶时间(TT)。结果环-(酪氨酸-亮氨酸)、环-(苯丙氨酸-苯丙氨酸)和环-(苯丙氨酸-酪氨酸)均可使大鼠血浆PT、APTT和TT显著延长(P<0.1)。前二者未呈现出量效关系,后者呈现正性量效关系。结论三棱的环二肽类成分均具有抗凝活性,以环-(苯丙氨酸-酪氨酸)的抗凝作用尤为显著。

AIM To study the in vitro anticoagulant activity of cyclodipeptide（ cyclo-（ Tyr-Leu）,cyclo-（ PhePhe） and cyclo-（ Phe-Tyr）） isolated from Sparganium stoloniferum Buch.-Ham. METHODS The special kits were used to measure anticoagulant activity with platelet poor plasma collected from abdominal aorta of male SD rats,including prothrombin time（ PT）,activated partial thromboplastin time（ APTT） and thrombin time（ TT）.RESULTS PT,APTT,TT were prolonged by the addition of cyclo-（ Tyr-Leu）,cyclo-（ Phe-Phe） and cyclo-（ Phe-Tyr）. The former two presented no dose-effect relationship but the latter did. CONCLUSION Of three cyclodipeptides from S. stoloniferum,all the three show anticoagulant activities with cyclo-（ Phe-Tyr） being by far the most effective.