绞股蓝对胆固醇诱导的非酒精性脂肪性肝病兔肝纤维化血清标记物的影响

Effects of Gynostemma Pentaphyllum on serum markers of liver fibrosis in cholesterol-induced non-alcoholic fatty liver disease in rabbits

目的：探讨绞股蓝对非酒精性脂肪性肝病（nonalcoholic fatty liver disease, NAFLD）兔肝纤维化血清标记物的影响。方法将40只成年雄性兔按随机数字表法随机分为绞股蓝组、辛伐他汀组、模型组、正常对照组，每组10只。正常对照组给予标准饲料喂养，其余动物给予高脂饲料100 g/d喂养，建立NAFLD模型。绞股蓝组灌胃绞股蓝水煎液5 g/（kg·d），辛伐他汀组灌胃辛伐他汀溶液5 mg/（kg·d），空白组及模型组灌胃等体积蒸馏水。连续给药9周。检测血清总胆固醇（TC）、三酰甘油（TG）、C-反应蛋白（CRP）、透明质酸（HA）、层黏连蛋白（LN）水平及肝组织TG水平。结果与正常对照组比较，模型组血清TG[（1.72±0.61）mmol/L比（0.85±0.39）mmol/L]、TC[（60.50±9.77）mg/L比（1.30±0.44）mg/L]、CRP[（256.79±30.78）mg/L比（8.71±1.41）mg/L]、HA[（798.8±69.4）ng/ml比（121.2±6.8）ng/ml]、LN[（964.8±62.8）ng/ml 比（142.4±12.2）ng/ml]显著升高（P＜0.01）。与模型组比较，绞股蓝组血清TG[（1.31±0.39）mmol/L 比（1.72±0.61）mmol/L]、TC[（36.44±6.57）mmol/L比（60.50±9.77）mmol/L]、CRP[（68.77±10.78）mg/L比（256.79±30.78）mg/L]、HA[（420.8±60.2）ng/ml比（798.8±69.4）ng/ml]、LN[（449.8±56.6）ng/ml比（964.8±62.8）ng/ml]以及肝组织TG[（0.52±0.10）mmol/L比（0.77±0.08）mmol/L]显著降低（P＜0.01）。绞股蓝组肝组织TG水平[（0.52±0.10）mmol/L比（0.59±0.09）mmol/L]较辛伐他汀组显著降低（P＜0.05），但血清TC、TG、CRP、HA和LN水平与辛伐他汀组比较，差异无统计学意义（P＞0.05）。结论绞股蓝可调节NAFLD兔血脂代谢，减轻炎症反应，降低肝纤维化血清血清标记物水平，可能对肝纤维化有保护作用。

Objective To investigate the effects of Gynostemma Pentaphyllum on serum markers of liver fibrosis in cholesterol-induced non-alcoholic fatty liver disease （NAFLD） in rabbits. Methods Forty adult male white rabbits were randomly divided into a normal control group, a model group, a treatment group and a simvastatin group, with 10 rabbits in each group. NAFLD was induced with a high-cholesterol diet. After modeling, the rabbits in the treatment group were intragastrically administrated with Gynostemma Pentaphyllum 5 mg/（kg·d）, the simvastatin group with simvastatin 5 mg/（kg·d）, and the model and normal control groups with the equal volume of distilled water for 9 weeks. The serum levels of total cholesterol （TC） and triglyceride （TG）, the serum inflammatory maker C-reactive protein （CRP）, the serum markers of liver fibrosis such as hyaluronic acid and laminin （LN）, and the TG level in the liver tissue were detected. Results Compared with the normal control group, the serum levels of TC （60.50 ± 9.77 mg/L vs.1.30 ± 0.44 mg/L）, TG （1.72 ± 0.61 mmol/L vs. 0.85 ± 0.39 mmol/L）, CRP （256.79 ± 30.78 mg/L vs. 8.71 ± 1.41 mg/L）, HA （798.8 ± 69.4 ng/ml vs. 121.2 ± 6.8 ng/ml）,LN （964.8 ± 62.8 ng/ml vs. 142.4 ± 12.2 ng/ml） in the model group were increased significantly （all P〈0.01）. Compared with the model group, the serum levels of TC （36.44 ± 6.57 mmol/L vs. 60.50 ± 9.77 mmol/L）, TG （1.31 ± 0.39 mmol/L vs. 1.72 ± 0.61 mmol/L）, CRP （68.77 ± 10.78 mg/L vs. 256.79 ± 30.78 mg/L）, HA （420.8 ± 60.2 ng/ml vs. 798.8 ± 69.4 ng/ml）, LN （449.8 ± 56.6 ng/ml vs. 964.8 ± 62.8 ng/ml） and the TG level in the liver tissue （0.52 ± 0.10 mmol/L vs. 0.77 ± 0.08 mmol/L） in the treatment group were decreased significantly （all P〈0.01）. The TG level in the liver tissue in the treatment group was significantly lower than that in the simvastatin group （0.52 ± 0.10 mmol/L vs. 0.59 ± 0.09 mmol/L;P〈0.05）. There were no significant difference in the serum levels of TC, TG,CRP,HA and LN between the treatment group and the simvastatin group （all P〉0.05）. Conclusions Gynostemma Pentaphyllum may regulate lipid metabolism, alleviate inflammation, and decrease serum markers of liver fibrosis, and might protect against liver fibrosis in rabbits with NAFLD.