氰乙基化废纸的生物降解性能的初步研究

Degradation property of a cyanoethylated wastepaper

采用微生物学的实验方法初步研究了氰乙基化废纸的生物降解性能,结果表明,氰乙基化废纸具有比废纸更佳的纤维素酶降解性,在里氏木菌纤维素酶降解192 h后,失重率为23.6%.氰乙基化废纸在枯草芽孢杆菌亚种Bacillus subtilis（subsp）和苍白杆菌属Ochrobactrum sp.的作用下具有较佳的生物降解性;在实验室最佳降解条件下：温度35℃、pH值11左右、接种量25%～30%、底物质量浓度50 g/L,降解菌降解氰乙基化废纸速度先增大后减小,在12～14 d后降解速度相对变缓;降解14 d氰乙基化废纸产生的还原糖质量浓度基本保持在0.030 mg/m L左右,失重率最高为29.57%.

The degradation property of cyanoethylated wastepaper（CSF） has been explored with mierobiolo- gy experiment method.The results show that the cyanoethylated wastepaper can be effectively degraded by cellulo- ses,and the degradation property is better than wastepaper.After 192 hours＇ degradation by Trichoderma reesei cellulose, the weightlessness rate of CSF can reach 23.6%.The research on degradation features of reveals that cy- anoethylated wastepaper can be effectively degraded by bacteria which are Bacillus subtilis （subsp） and Ochrobactrum sp.Under the optimum conditions 35 ℃, pH 11.0,25%-30% inoculum size and 50 g/L substrate concentration, degradation speed of cyanoethylated wastepaper can be firstly increased and then reduced; Degra- dation speed is lower after 12—14 days.After degradation by bacterium for 14 days ,the content of reducing sugar remains about 0.030 mg/mL, and the weightlessness rate of cyanoethylated wastepaper is 29.57%