黑麦草老化处理后种子活力和生理生化特性的变化

Change of Seed Vigor and Physiological and Biochemical Characteristics of Barley with Artificial Aging Treatment

为延长黑麦草的种子寿命及其安全贮藏,以黑麦草邦德为试验材料,采用45℃,90%相对温度的条件进行人工老化处理,并对其种子发芽指标、相对电导率、丙二醛含量、可溶性糖、可溶性蛋白、POD和CAT活性等生理生化指标进行了测定。结果表明:相对电导率、可溶性糖和丙二醛含量等随着老化时间的延长逐渐升高,可溶性蛋白、POD和CAT活性等逐渐降低,表明黑麦草种子耐贮藏的适宜老化时间为3d。通过研究可知,在大麦种子的老化过程中,相对电导率、可溶性糖含量、可溶性蛋白质含量、POD和CAT活性均与种子的老化及劣变密切相关,可据此检测种子活力。

In order to prolong the life of perennial ryegrass seeds and safe storage ,with ryegrass Bond as experi‐mental material ,using 45℃ ,90% RH conditions of artificial aging treatment ,and the seed germination index , relative conductivity ,MDA content ,soluble sugar ,soluble protein ,POD and CAT activity and so on physiologi‐cal and biochemical indexes were determined .The results showed that the relative electrical conductivity ,solu‐ble sugar and MDA content increased with aging time prolong ,such as soluble protein ,POD and CAT activity gradually reduced ,suggests that the suitable aging time of perennial ryegrass seed storage tolerance for 3 d . Through research showed that in the process of barley seed aging ,relative electrical conductivity ,soluble sugar content ,soluble protein content ,POD and CAT activities were closely related with seed aging and deteriora‐tion ,shall be tested on the basis of seed vigor .