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患病细鳞鱼杀鲑气单胞菌的分离与鉴定 被引量:13

Isolation and identification of Aeromonas salmonicida from diseased Brachymystax lenok
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摘要 从患病细鳞鱼(Brachymystax lenok)的病变组织处分离到1株致病菌,经过分离培养,生化鉴定,16 S rRNA序列分析和人工感染实验确定该病原菌为杀鲑气单胞菌无色亚种(Aeromonas salmonicida subsp.achromogenes)。采用20种药物进行药敏分析,结果显示:分离菌株对阿米卡星、哌拉西林、恩诺沙星等9种抗生素敏感;对环丙沙星、诺氟沙星等4种抗生素中度敏感;对卡那霉素、青霉素、氨苄西林等7种抗生素耐药。 The pathogenic strain was isolated from diseased Brachymystax Lenok.Diagnosis was based on staining micros -copy, biochemical identification, 16SrRNA sequence analysis and animal experiment .The isolated bacteria was identified as Aeromonas salmonicida subsp achromogenes.The drug sensitivity test of a total of 20 antimicrobial drugs Showed that the isolated strain was sensitive to 9 kinds of drugs including amikacin , piperacillin, enrofloxacin, etc., and moderately sen-sitive to ciprofloxacin, norfloxacin etc.The strain was resistant to 7 kinds of antibiotics including kanamycin , penicillin and ampicillin etc.
出处 《淡水渔业》 CSCD 北大核心 2015年第1期88-92,共5页 Freshwater Fisheries
基金 北京市农业局科技项目(PXM2014_036332_000003_00130031_FCG) 云南省优势特色重点学科生物学一级学科资助
关键词 细鳞鱼(Brachymystax lenok) 杀鲑气单胞菌(Aeromonas salmonicida) 分离 鉴定 Brachymystax lenok Aeromonas salmonicida isolation identification
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