己酮可可碱对高位脊髓损伤大鼠心功能紊乱的影响

Effects of pentoxifylline on cardiac dysfunctions following high level spinal cord injury in rats

目的研究己酮可可碱(PTX)对大鼠高位脊髓损伤(SCI)心功能紊乱的影响及其可能的机制。方法采用Allens打击法建立中度高位SCI大鼠模型。术后腹腔注射PTX(30mg/kg),每12h1次。术后4、12、24、48和72h行颈动脉插管测定动脉血压(AP)、平均动脉压(MAP)、心率(HR),行左心室腔插管测定左心室收缩峰压(LVSP)、左心室压力最大上升速率(LVdP/dtmax)。全自动化学分析仪测定血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)乳酸脱氢酶(LDH)含量。紫外分光光度法测定心肌组织匀浆上清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果 SCI术后4、12、24、48和72h大鼠MAP、HR、LVSP和LVdP/dtmax均显著降低,心肌组织SOD活性均显著降低,MDA含量均显著升高,血清CK和CK-MB显著升高;血清LDH水平均显著升高。PTX治疗显著阻止上述改变。结论 PTX对SCI大鼠心功能具有保护作用,其机制涉及降低心肌氧化应激水平。

Objective To investigate the influnces of pentoxifylline （PTX） on cardiac dysfunctions after high level spinal cord injury （SCI） in rats .Methods The moderate high level SCI animal models were made using an improved Allens weight -drop device .The PTX was administered intraperitoneally （30 mg/kg） immediately after surgery ,and then once every 12 hours .At 4 , 12 ,24 ,48 and 72 h ,arterial pressure （AP） ,mean arterial pressure （MAP） and heart rate （HR） were determined with a catheter placed into the carotid artery .And then left ventricular systolic pressure （LVSP） and left ventricular peak velocities of contraction （LVdP/dtmax） were measured with a catheter placed into left ventricle .Serum creatine kinase （CK） ,CK-MB and lactate dehydrogenase （LDH） were measured using automatic chemical analyzer .Superoxide dismutase （SOD） activity and malondialdehyde （MDA） level in the myocardium tissues were determined by ultraviolet spectrophotometry method .Results SCI induced significantly lower MAP ,HR ,LVSP and LVdP/dtmax ,and lower SOD activity and higher MDA level in the myocardium tissues ,as well as higher serum CK and CK-MB level .At 12 ,24 ,48 and 72 h after surgery ,SCI induced significantly higher serum LDH level .At 4 ,12 and 24 h after surgery ,PTX treatment significantly prevented the above changes in SCI rats .Conclusions PTX had protective effect on heart function in high level SCI rats ,and its mechanism involved reduced oxidative stress of the myocardium tissues .