血液感染新生儿血培养病原菌及药敏试验分析

The analysis of pathogens and their drug susceptibility from neonates with blood infection

目的分析新生儿血培养阳性标本中各病原菌分布情况及主要病原菌的耐药特点。方法采用BACTEC-9120全自动血培养仪,BD专用儿童体液培养瓶培养血液样本,分离所得菌株采用VITEK32自动微生物分析仪进行细菌鉴定和药物敏感试验。结果血培养阳性结果以革兰氏阳性菌为主,占74.8%,其中以表皮葡萄球菌为主;革兰氏阴性杆菌占24.2%。革兰氏阳性菌对万古霉素均敏感;革兰氏阴性杆菌对碳青霉烯类抗菌药物高度敏感。结论病原菌耐药性检测对血液感染新生儿非常重要,根据药敏结果选用敏感抗菌药物,可提高临床治疗效果,减少细菌耐药性的产生。

Objective To investigate the distribution and antibiotic resistance of pathogens isolated from blood specimens of neonates .Methods The BACTEC9120 automatic blood culture instrument and BD special children fluid culture bottle were used to culture the blood of neonates .Isolated strains were identified ,and their drug sensitivity tests were completed by VITEK 32 system .Results The results showed that Grampositive cocci accounted for 74.8% ,in which Staphylococcus epidermidis ac counted mainly ,and gramnegative bacteria accounted for 24.2% .grampositive cocci were sensitive to vancomycin ,and gram negative bacteria were sensitive to carbapenem .Conclusion Pathogen drug resistance detection is very important for neonates with blood infection .Choosing sensitive antibiotic drugs according to the result of drug susceptibility can improve the clinical curative effect and reduce the antibiotic resistance .