P53siRNA对肺腺癌细胞A549中HMGN2表达的影响

The Influence of P53siRNA on the HMGN2 Expression in Lung Adenocarcinoma Cancer Cells A549

目的探讨P53基因调节人肺腺癌细胞A549中HMGN2蛋白表达情况。方法采用小分子干扰RNA(P53-siRNA)真核细胞转染人肺腺癌细胞株A549作为实验组P53-si,转染无荧光RNA的A549细胞为空白对照组con-si,未转染任何载体的A549细胞为正常对照组con。Western blot检测HMGN2和P53表达的变化。结果转染P53-siRNA的实验组A549细胞株中HMGN2蛋白表达下调,空白对照组和正常对照组A549细胞中HMGN2的表达均无明显变化;相对于正常对照组,HMGN2的表达率分别为:实验组56%,空白对照组88%(P<0.01)。结论 P53基因能下调人肺腺癌细胞中HMGN2的活性,参与调控HMGN2蛋白的表达,从而为P53基因参与调节HMGN2在小鼠发育过程的机制研究提供实验基础。

Objective To explore the influence of p53 on the expression of the HMGN2 in human lung adenocarcinoma cell line A549.Methods Two lines of A549 cells transfected by P53-siRNA（P53-siRNA）,were established as experimental groups（P53-si）.One line of A549 cells transfected by no fluorescence RNA served as black normal controls group（con-si）and A549 cells transfected by nothing as controls group（con）.The expressions of p53 mRNA and HMGN2 protein were detected by Western blot analysis.Results The expression of HMGN2 protein were down-regulated in the P53-si group,while HMGN2protein＇s expressions in the other two groups had no obvious change.HMGN2protein＇s expression rates were 78% in P53-siRNA group and 95% in con-si group（P0.01）.Conclusion P53 gene can lower the activity of HMGN2 in human lung adenocarcinoma cell,and that regulating the expression of HMGN2 proteins can really help the P53 gene involved in mediation HMGN2 in mice development mechanism research provide the experimental base.