免疫性血小板减少症患者协同刺激分子的表达及意义

The Expression and Significance of Cosimulatory Molecules in Peripheral Blood Lymphocytes of Patients with Immune Thrombocytopenia

目的:探讨特发性血小板减少性紫癜(ITP)患者外周血淋巴细胞CD28、CD80及CD86的表达及意义。方法:采用免疫荧光标记和流式细胞术检测30例ITP患者和30例健康对照者外周血CD28细胞、CD80细胞和CD86细胞分别占淋巴细胞的比例及血小板表面相关抗体水平,并进行对比、分析。结果:与正常对照组相比,ITP患者外周血CD28细胞增多,差异有统计学意义(P<0.05);CD80细胞增多,但差异无统计学意义(P>0.05);CD86细胞显著增多,差异有统计学意义(P<0.05),此结果与ITP是否急慢性无关。治疗前后患者的CD28、CD80及CD86的表达率比较差异无统计学意义(P>0.05)。患者与正常对照组PAIg A、PAIg G和PAIg M的水平比较差异有统计学意义(P<0.05);CD80、CD86表达与PAIg G水平之间存在显著的相关性,比较差异有统计学意义(P<0.01)。结论:ITP患者外周血淋巴细胞CD28、CD80及CD86表达均异常,可能与其发病相关。

Objective： To investigate the expression and significance of costimulatory on peripheral lymphocytes of CD28, CD80 and CD86 in patients with immune thrombocytopenia （ ITP ） .Method： Blood samples were collected from 30 ITP patients and 30 healthy controls.The percentages of CD28, CD80 and CD86 on lymphocytes and platelet associated immunoglobulins （ PAIg ） were compared and anzlysed with flow cytometry and monoclonal antibodies technology. Result： The percentage of CD28 was higher in ITP patients than those in lontrols, the difference was statistically significant （ P〈0.05 ） .higher proportions of CD80 in ITP patients than those in controls, but there was not statistically significant （ P〉0.05 ） .The percentage of CD86 in ITP patients was higher than in controls, this result had nothing to do with ITP whether acute or chronic.Before and after treatment in patients with the expression of CD28, CD80 and CD86 had no statistically significant differences （ P〉0.05 ） .The levels of PAIgA, PAIgG and PAIgM in ITP patients were higher than those in controls. The expression of CD80 and CD86 had positive correlation with the level of PAIgG, the differences were statistically significant （ P〈0.01 ） .Conclusion： The abnormal expression of costimulatory on peripheral lymphocytes of CD28, CD80 and CD86 in patients may be related to the pathogenesis of ITP.