促红细胞生成素对大鼠脑缺血再灌注后血脑屏障通透性和ZO-1表达的影响被引量：3

Effects of erythropoietin on the permeability of blood brain barrier and the expression of ZO-1 in cerebral ischemia-reperfusion rats

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摘要目的研究促红细胞生成素(erythropoietin,EPO)预处理对大鼠脑缺血再灌注后血脑屏障通透性和紧密连接蛋白ZO-1的影响。方法采用大鼠大脑中动脉栓塞(MCAO)模型,伊文思蓝渗透性实验检测血脑屏障通透性,免疫组化法和Western blot法测定ZO-1蛋白表达,RT-PCR法测定ZO-1 mRNA表达。结果 EPO预处理能够明显减轻脑缺血再灌注后血脑屏障的通透性(P<0.01),并显著上调缺血脑组织中ZO-1蛋白及mRNA的表达水平(P<0.01)。结论 EPO通过上调脑缺血再灌注后脑组织中ZO-1的表达,降低BBB通透性,这可能是EPO保护脑缺血再灌注后神经组织的机制之一。 Objective To study the effects of erythropoietin （EPO） pretreatment on blood brain barrier （BBB） and ZO-1 expression after cerebral ischemia-reperfusion in rats. Methods Using middle cerebral artery occlusion （MCAO） model in rats, the BBB permeability was assessed by Evans blue extravasation. The changes of ZO-1 protein and mRNA were determined by immunohistochemistry assays, Western blot, and reverse transcriptase-polymerase chain reaction, respectively. Results EPO significantly reduced BBB permeability（P 〈 0.01 ） and markedly increased the expressions of ZO-1 protein and mRNA in ischemia tissue after cerebral ischemia-reperfusion （ P 〈 0.01 ）. Conclusion EPO could decrease the BBB permeability by increasing the expression of ZO-1 ,and which may be one of the protection mechanism of EPO on brain tissue after cerebral ischemia-reperfusion.

作者刘坤姚阳商丽宏徐畅薛一雪

机构地区沈阳医学院生理教研室沈阳医学院临床医学系中国医科大学神经生物学教研室

出处《中风与神经疾病杂志》 CAS CSCD 北大核心 2014年第12期1064-1067,共4页 Journal of Apoplexy and Nervous Diseases

基金沈阳市科技局计划项目(F10-205-1-21)

关键词脑缺血再灌注血脑屏障促红细胞生成素 ZO-1 Cerebral ischemia reperfusion Blood brain barrier Erythropoietin ZO-1

分类号 R743.3 [医药卫生—神经病学与精神病学]

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