人参对脊髓损伤大鼠MDA、SOD、NO含量的影响及意义

Influence and significance of ginseng on the levels of MDA、SOD and NO for SCI rats

目的探讨人参对脊髓损伤后脊髓组织中MDA、SOD、NO含量的影响及意义。方法将72只成年雄性Wistar大鼠随机分成3组,每组24只。SCI损伤组:做SCI手术,不进行治疗;人参治疗组:SCI后,立即服用人参超微粉碎颗粒;假手术组:只打开椎板,暴露脊髓,不造成SCI。每组根据取材时间进一步分为术后0.5 h、2 h、4 h、6 h 4小组,每小组6只,测定脊髓损伤处MDA、SOD、NO含量。结果 SCI损伤组与治疗组MDA、SOD及NO含量术后0.5 h组未见差异,而术后2 h、4 h和6 h组显示治疗组对脊髓损伤后MDA升高有明显抑制作用,而对SOD含量降低有显著的回升作用,NO含量偏低,差异有统计学意义(P<0.01)。结论人参在脊髓损伤后能够有效并及早的保护脊髓组织,减轻脊髓继发性损伤。

Objective To explore the effect and significance of Ginseng on the levels of MDA,SOD and NO on myeloid tissue for rats with spinal cord injury. Methods Seventy two male Wistar rats were randomly assigned to 3 groups （ n = 24 per group）. SCI group： No treatment after SCI was created. Ginseng group： Ginseng treatment after SCI immediately. Sham group：neural seute was opened and spinal cord was exposed without SCI. Every group was assigned to 4 subgroups（ n = 6 per subgroup） according to the time of the tissue was used,including 0.5 h,2 h,4 h and 6 h subgroups, for the levels determination of MDA,SOD and NO on the region of SCI. Results There were no obviously difference on the levels of MDA,SOD and NO between SCI group and Ginseng group after SCI 0.5 h. Ginseng group was observed the increase of MDA and the decrease of SOD were restrained obviously and the levels of NO was lower in Ginseng group than in SCI group after SCI 2 h,4 h and 6 h. Difference between Ginseng group and SCI group was statistically significant （P 〈 0. 01 ）. Conclusion Ginseng can protect the myeloid tissue early and effectively after SCI and then to relieve the secondary injuries following spinal cord trauma.