儿童注意分散度与多巴胺D2受体基因rs1079727多态性的关系

Association between rs1079727 polymorphism in the DRD2 gene and the distractibility in the school-age children

目的 了解多巴胺D2受体（DRD2）基因rs1079727多态性与学龄儿童注意分散度的关系.方法 运用Carey 8～ 12岁MCTQ儿童气质问卷、自动化MassArray SNP基因型分型技术、单因素方差分析和Logistic回归分析法,分析121例8～12岁儿童的注意分散度与DRD2基因rs1079727多态性的相关性,重点探讨了性别、分娩方式与基因交互作用对注意分散度的影响.结果 DRD2基因rs1079727多态性、分娩方式均与儿童注意分散度明显相关,GG和AG基因型儿童的注意分散度分值[分别为（4.37±0.53）分,（4.27±0.69）分]高于AA基因型儿童（4.01±0.67）分,剖宫产儿童注意分散度高分组比例（48.28％）高于顺产儿童（33.90％）,均差异有统计学意义（P＜0.05）;rs1079727多态性与性别、rs1079727多态性与分娩方式的交互作用均对儿童注意分散度有影响,G等位基因会增加女孩发生注意分散的风险（OR值为2.231,P＜0.05）,顺产时A等位基因对儿童注意分散度具有一定保护作用（OR值为0.048,P＜0.01）,差异均有统计学意义.结论 分娩方式、rs1079727多态性及其与性别/分娩方式的交互作用共同作用于注意分散度.

Objective To understand the association between rs1079727 polymorphism in the DRD2 gene and the distractibility in the school-age children.Method The rs1079727 polymorphisms were genotyped using Sequenom Mass Array and the distractibility was measured based on the Middle Childhood Temperament Questionnaire （MCTQ） in 121 children aged from 8 to 12 years,in order to analyze the function of rs1079727 polymorphism and its interaction with gender or the delivery mode on the distractibility.The results were analyzcd with oneway ANOVA and logistic regression analysis.Results There was association between the distractibility and rs1079727 polymorphism or delivery mode respectively.The means of distractibility for the genotypes of GG （4.37 ±0.53） and AG （4.27±0.69） were significantly higher than that of the genotype of AA（4.01±0.67） （P〈0.05）.The proportion of high distractibility in the cesarean children （48.28%） was significantly higher than that in the natural labor children （33.90%） （P〈0.05）.The child distractibility occurred through an interaction between rs1079727 polymorphism and gender/delivery mode.The girls with G alleles possessed increased risk of distractibility （OR=2.231,P〈0.05）,and the natural labor children with A alleles were tend to be associated with low distractibility（OR =0.048,P〈0.01）.Condusion The distractibility based the temperament for the school-age children might be influenced by delivery mode,rs1079727 polymorphism and its interaction with gender/delivery mode.