舒心胶囊对大鼠血脂及血小板聚集的影响

Impacts of Shuxin Capsules on Blood Lipid and Platelet Aggregation in the Rats

目的研究舒心胶囊对高脂饲料诱导的大鼠高脂血症模型动物血脂及血小板的影响。方法 120只Wistar大鼠除了正常对照组20只外,其余100只喂养脂肪乳20 d后,采血测定血清总胆固醇浓度,根据总胆固醇值,随机分成模型组、舒心胶囊大剂量组(2.4 g·kg-1)、舒心胶囊中剂量组(1.2 g·kg-1)、舒心胶囊低剂量组(0.4 g·kg-1)和阳性对照组(复方丹参滴丸组,90 mg·kg-1),每组20只。每日灌胃给药或生理盐水1次,连续14周并继续喂以脂肪乳,实验前1 d各组大鼠禁食12 h,实验当天将动物用戊巴比妥钠麻醉,腹主动脉采血,肝素抗凝,高速离心机分离血清,测血清清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)含量及血小板聚集程度。结果 14周后模型组血清TC、TG,LDL-C均高于正常对照组,HDL-C低于正常对照组,表明造模成功。舒心胶囊大、中、小剂量组与模型组比较,TC、TG、LDL-C显著下降(P<0.05,P<0.01),而HDL显著升高(P<0.05,P<0.01)。舒心胶囊大、中、小剂量组,复方丹参滴丸的血小板最大聚集率与正常对照组比较差异有统计学意义。结论舒心胶囊具有降低血脂和改善血小板功能的作用。

Objective To study the impacts of shuxin capsules on blood lipid and platelet in hyperlipemia induced by high- fat diet. Methods Among 120 Wistar rats,20 rats were in the normal control group. After fed with fat milk for 20 days,blood was collected in the rest 100 rats to determine the concentration of serum total cholesterol（ TC）. According to TC level,they were randomized into a model group,a large- dose group（ 2. 4 g·kg^-1,shuxin capsules）,a middle- dose group（ 1. 2 g·kg^-1,shuxin capsules）,a low- dose group（ 0. 4 g·kg^-1,shuxin capsules） and a positive control group（ 90 mg·kg^-1,compound danshen dropping pills）,20 rats in each group. Medication with gastric perfusion or physical saline was applied once a day,continuously for 14 weeks. Additionally,the fat milk was kept on feeding. One day before the test,the rats in each group were fasting for 12 h. On the day of the test,the rats were anesthetized with pentobarbital sodium. Blood was collected from aorta abdominalis,heparin was used for anticoagulation and the high speed centrifuge for serum separation. TC,TG,HDL- C and LDL- C levels as well as platelet aggregation degree were determined. Results In 14 weeks,TC,TG and LDL- C levels in the model group were higher than those in the normal control group,HDL- C level was lower than that in the normal control group,indicating the modeling success. Compared with those in the model group,TC,TG and LDL- C levels were reduced significantly in the large- dose group,middle- dose group and small- dose group（ P〈0. 05,P〈0. 01）,and HDL level was increased significantly（ P〈0. 05,P〈0. 01）. In the large- dose group,middle- dose group and small-dose group,the maximal aggregation rate of platelet in a positive control group treated with compound danshen dropping pills was different significantly as compared with that in the normal control. Conclusion Shuxin capsules reduce blood lipid and improve platelet function.