斑蝥酸钠对体外培养肺癌患者淋巴细胞免疫应答的影响

Effect of disodium cantharidinate on in vitro lymphocyte immune response in lung cancer patients

目的 探讨斑蝥酸钠对体外培养肺癌患者淋巴细胞免疫应答的影响.方法 病理诊断为非小细胞肺癌的患者20例,体外进行人外周血淋巴细胞培养,应用四甲基偶氮唑盐法检测不同浓度斑蝥酸钠维生素B6作用下淋巴细胞的增殖影响情况,应用流式细胞仪分析不同浓度斑蝥酸钠对CD4^＋ CD25^＋T细胞、CD25^＋ FOXP3^＋T细胞、CD4^＋和CD8^＋T细胞的影响.结果 当斑蝥酸钠维生素B6注射液浓度＜10 μg/ml时,浓度升高能刺激外周血淋巴细胞的增殖.斑蝥酸钠维生素B6浓度＞lOμg/ml,随着浓度的升高,斑蝥酸钠对淋巴细胞增殖的刺激作用减弱.当斑蝥酸钠维生素B6注射液浓度＜10 μg/ml时,随着实验组浓度的升高,与对照组相比,CD4^＋/CD8^＋比值明显升高（t=2.171,P=0.032;t=2.103,P=0.041;t=3.662,P =0.002;t =3.201,P=0.003;t =3.233,P =0.003）,CD4^＋ CD25^＋/CD4^＋和CD25^＋ FOXP3^＋T明显降低,差异有统计学意义;但当斑蝥酸钠维生素B6注射液浓度高于10 μg/ml时,斑蝥酸钠10、15、20 μg/ml组CD4^＋/CD8^＋比值、CD4^＋ CD25^＋/CD4^＋和CD25^＋ FOXP3^＋T变化均不大.结论 斑蝥酸钠维生素B6在一定范围内能促进体外培养肺癌患者淋巴细胞的增殖,并提高其细胞免疫能力.

Objective To investigate the effect of disodium cantharidinateon in vitro lymphocyte immune response in lung cancer patients.Methods Twenty non-small cell lung cancer patients diagnosed by pathological diagnosis were selected.The effects of different concentrations of disodium cantharidinate and vitamin B6 on lymphocyte proliferation and CD4^＋ CD25^＋ T cells,CD25^＋ FOXP3^＋ T cells,CD4^＋ and CD8^＋ T cells were detected by methyl thiazolyl tetrazolium assay and flow cytometry.Results When the concentrations of disodium cantharidinate and vitamin B6 was less than 10 μg/ml,with the growth of concentration,it stimulated the proliferation of peripheral blood lymphocytes.When the concentration was more than 10 μg/ml,with the growth of concentration,the absorbance value decreased,and the stimulating effect weakened.When the concentration of disodium cantharidinate and vitamin B6 injection was less than 10 μg/ml,with the growth of concentration,CD4^＋/CD8^＋ ratio increased significantly,and CD4^＋ CD25^＋/CD4^＋ and CD25^＋ FOXP3^＋ T cells were significantly lower,which showed statistically significant differences （t =2.171,P =0.032 ; t =2.103,P =0.041 ; t =3.662,P =0.002 ; t =3.201,P =0.003 ; t =3.233,P =0.003）.But when the concentration was more than 10 μg/ml,The differences of CD4^＋/CD8^＋ ratio,CD4^＋ CD25^＋/CD4^＋ and CD25^＋ FOXP3^＋ T cells in 10,15,20 μg/ml groups were not statistically significant,which showed that disodium cantharidinate and vitamin B6 injection had a positive effect on enhancing the immune function of lymphocytes in lung cancer in a concentration dependent manner,but high concentration was unhelpful.Conclusion Disodium cantharidinate and vitamin B6 injection can promote lung cancer in vitro lymphocyte proliferation as well as increase its immunity in a certain range.