Ion Torrent测序检测非小细胞肺癌外周血DNA的临床价值

The Clinical Value of Ion Torrent Sequencing with DNA for Non- small Cell Peripheral Lung Cancer

目的:探讨Ion Torrent测序检测非小细胞肺癌外周血DNA的临床价值。方法:选择患者80例,分为两组,各40例,观察组利用三代测序技术Ion Torrent检测,对照组使用第1代测序技术Sanger末端终止法进行测序,比较手术前后通过Ion Torrent测序所得EGFR和K-ras结果,并统计两组测序EGFR阳性1年生存情况。结果:未手术者观察组EGFR阳性和K-ras突变阳性检出率均显著高于对照组(P<0.05),术后复发患者观察组EGFR阳性和K-ras突变阳性检出率均显著高于对照组(P<0.05),观察组1年生存率显著高于对照组(P<0.05)。结论:对非小细胞肺癌患者使用Ion Torrent测序技术测定外周血DNA,能更准确的确定患者基因类型,从而为分子靶向治疗提供依据。

Objective：To investigate the clinical value of ion torrent sequencing with DNA for non -small cell peripheral lung cancer .Method：80 cases were divided into two groups , each with 40 cases, the observation group were used three generations with ion torrent sequencing technology ,the control group were used the first-generation sequencing technology method with Sanger sequencing , then sequencing proceeds before and after surgery and EGFR by ion torrent K-ras results and statistics sequencing EGFR-positive a two-year survival were compared .Result：The observation group with no surgery of EGFR-positive and K-ras mutation positive detection rate were significantly higher than those of control group （P〈0.05）, the ob-servation group with recurrence of EGFR-positive group and K-ras mutations were significantly higher than those of control group （ P〈0.05） , 1-year survival rate in the observation group was significantly higher than that of control group （P〈0.05）.Conclusion：Ion torrent sequencing technology to measure peripheral blood DNA with non-small cell lung cancer patients can more accurately determine the genetic type of the patient , and thus provide the basis for molecular targeted therapy .