乳腺癌髓系来源抑制细胞中吲哚胺2,3-双加氧酶表达与Tregs分布关系及临床意义

Relationship and Clinical Significance between Myeloid Suppressor Cells in Indoleamine 2,3- Expression and Distribution of Tregs Dioxygenase in Breast Cancer

目的:研究乳腺癌CD33+髓系来源的抑制细胞(MDSCs)中吲哚胺2,3-双加氧酶(IDO)表达情况及与Foxp3+调节性T细胞(Tregs)分布关系和临床意义。方法:运用免疫组化单染技术检测CD33+MDSCs细胞、Foxp3+Tregs,采用免疫组化双染检测CD33+MDSCs细胞中IDO的表达情况。结果:癌组织中CD33+MDSCs细胞、Foxp3+Tregs分布无序,呈散在分布;Foxp3+Tregs高比例组中,CD33+MDSCs细胞中IDO呈高表达,差异有统计学意义(P<0.05);CD33+MDSCs中IDO的表达与患者年龄、肿瘤直径和组织学分级无明显关系,但与是否有淋巴结转移有密切关系(P<0.05)。结论:乳腺癌中CD33+MDSCs中IDO高表达参与肿瘤细胞免疫耐受的形成,可能与乳腺癌淋巴转移存在密切联系。

Objective：To study the expression of indoleamine 2,3-dioxygenase （ IDO） in CD3^3＋mye-loid-derived suppressor cells （ MDSCs） and the relationships between the expression of IDO and Foxp^3＋reg-ulatory T cells （ Tregs） distribution and clinical significance. Method：Immunohistochemical single staining technique was used to detect CD3^3＋MDSCs cells and Foxp3＋Tregs.The immunohistochemical double staining was used to detect CD3^3＋MDSCs cells indoleamine 2,3-bis dioxygenase （IDO） expression.Result：Carci-noma CD3^3＋MDSCs cells, Foxp3＋Tregs distribution were disorder and scattered in carcinoma. It showed sig-nificant difference between the expression of MDSCs IDO and Tregs distribution （ P〈0.05）. The expression of CD3^3＋MDSCs in IDO had no relation with breast cancer , irrespective of age , tumor size and histological grade were coreelated with whether lymph node metastasis （P〈0.05）.Conclusion：It showed a close contact between carcinoma CD 33＋MDSCs IDO expression in tumor cells and the formation of immune tolerance. Carcinoma CD3^3＋MDSCs IDO expression may be associated with the presence of lymph node metastasis.