RP-HPLC法同时测定葡萄酒、果酒、果醋中16种单体酚的含量

Simultaneous Determination of the Contents of 16 Types of Monophenols in Wine, Fruit Wine, and Fruit Vinegar by RP–HPLC

新建一种同时适用于天然果蔬饮品葡萄酒、果酒、果醋中8种酚酸、4种黄烷-3-醇、3种黄酮醇、1种芪类含量测定的HPLC分析方法。使用Waters XBridgeTM Shield RP18(4.6×250 mm,3.5μm)色谱柱,乙腈-冰乙酸-水溶液为流动相,流速0.8 m L/min,进样量20 u L,梯度洗脱,柱温30.0℃,DAD检测器同时采集280 nm和320 nm下的信号。16种单体酚均可有效分离,各物质的含量与峰面积线性关系良好,且回收率高,重复性好,具有定量准确、快速等特点。运用该方法测定了3种葡萄酒、3种果酒、3种果醋中16种单体酚的含量。结果表明,干红葡萄酒、干白葡萄酒、桃红葡萄酒能被定量的单体酚分别有14种、14种、9种单体酚;苹果酒中能被定量的单体酚有7种,樱桃酒7种,石榴酒8种;苹果醋中能被定量的单体酚有8种,柿子醋9种,猕猴桃醋中8种。该法准确、可靠,可同时适于不同天然果蔬饮品葡萄酒、果酒、果醋中酚类物质含量的分析。

A new method based on HPLC was established for the simultaneous determination of the contents of eight types of phenolic acids, four types of flavan-3-alcohols, three types of flavonols, and one type of stilbene in natural fruit and vegetable products, including wine, fruit wine, and fruit vinegar. Chromatographic separation was performed using a Waters XBridgeTM Shield RP18 （4.6×250 mm, 3.5 μm） column at 30 ℃ with an acetonitrile/acetic acid/water mixture as the mobile phase at a flow rate of 0.8 mL/min. The injection volume was 20 μL; gradient elution was applied and a diode array detector （DAD） was used to monitor signals at 280 μm and 320 urn. All 16 monophenols were successfully separated and their contents showed good linear relationships with the peak areas. The method had a high recovery rate, good repeatability, good accuracy, and high speed. The contents of 16 types of monophenols in three wines, three fi＇uit wines, and three fi＇uit vinegars were determined using this method. The results suggested that fourteen, fourteen, and nine types of monophenols could be quantified in dry red wine, dry white wine, and ros6 wine, respectively; seven, seven, and eight types could be quantified in apple wine, cherry wine, and pomegranate wine, respectively; eight, nine, and eight types could be quantified in apple vinegar, persimmon vinegar, and kiwifi＇uit vinegar, respectively. The results indicated that the method was accurate and reliable, and was suitable for the analysis of different monophenols in various fruit and vegetable drinks including wine, fi＇uit wine, and fruit vinegar.