固相微萃取-气质联用分析贵州沙姜挥发性成分

Analysis of Volatile Components of Kaempferia galanga L. from Guizhou by Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry

本文采用固相微萃取-气质联用技术分析了贵州沙姜的挥发性成分,同时,采用相应技术及傅立叶变换红外将所得主要成分与广西沙姜的成分进行了比较。结果发现:贵州沙姜的挥发性成分不同于广西沙姜,主要为爱草脑,其次是2,4,6,7,8,8a-六氢-3,8-二甲基-4(1-甲基亚乙基)-5(1H)-薁酮和塞舌尔烯。采用峰面积归一化法,对匹配度大于90%的可检索组分进行了统计和分析,结果表明:从贵州沙姜中鉴别出的挥发性成分共47个,三主要成分含量占总体的绝大多数,爱草脑、2,4,6,7,8,8a-六氢-3,8-二甲基-4(1-甲基亚乙基)-5(1H)-薁酮和塞舌尔烯的含量分别为61.53%、15.54%和7.19%;鉴别出的47个挥发性成分的分布是醚类4个、酮类3个、萜类28个、酚类3个、醇类2个、酯类2个、醛类2个、烃类3个,其中醚类、酮类、萜类化合物的含量较高,分别为62.22%、15.83%、13.68%。

The volatile components ofKaempferia galanga L. from Guizhou were analyzed using gas chromatography-mass spectrometry （GC-MS） coupled with solid phase microextraction （SPME）. The volatile components were also compared with those ofKaempferia galanga L. from Guangxi using the same techniques in combination with Fourier transform infrared （FT-IR） spectroscopy. The volatile components of Kaempferia galanga L. from Guizhou were different from those from Guangxi. The main components were estragole, followed by 5（1H）-azulenone, 2,4,6,7,8,8a-hexahydro-3,8-dimethyl-4-（1-metbylethylidene）, and seychellene. Using the peak area normalization method, the detectable components with over 90% matching were statistically analyzed. Notably, 47 volatile components were detected in Kaempferia galanga L. from Guizhou, and the three major components comprised most of the contents： estragole at 61.53%, 5（1H）-azulenone, 2,4,6,7,8,8a-hexahydro-3, 8-dimethyl-4-（1-methylethylidene） at 15.54%, and seychellene at 7.19%. Moreover, the 47 identified volatile components included ethers （4）, ketones （3）, terpenoids （28）, phenolic compounds （3）, alcohols （2）, esters （2）, aldehydes （2）, and hydrocarbons （3）. Among them, ethers, ketones, and terpenoids comprised 62.22%, 15.83%, and 13.68% of the total composition, respectively.