豆渣石油醚萃取物的FT-IR和GC/MS分析

Analysis of Petroleum Ether Extract from Soybean Dreg by FT-IR and GC/MS

为了解豆渣中小分子物质的赋存形态,本研究用石油醚作溶剂在索式萃取器中对豆渣进行了萃取,并采用傅里叶转换红外光谱仪(FT-IR)和气相色谱/质谱联用仪(GC/MS)对所得石油醚萃取物进行分析,同时用FT-IR对原料豆渣和萃余残渣也进行了分析。结果表明:实验连续萃取5 h,所得石油醚萃取物为淡黄色油状物,萃取率为10.7%;原料和萃余残渣的红外谱图峰形相近,但各吸收峰的吸收强度有所不同,而石油醚萃取物与原料和萃余残渣的红外谱图有明显的差别;石油醚萃取物中共检测到35种GC/MS可识别小分子有机化合物,主要分为烷烃、烯烃、芳香烃、醛、酸、酯及其它类物质7种类别;其中,酯类化合物相对含量为最多,高达61.79%。该研究在开发豆渣的高附加值利用方面具有重要的基础理论意义。

In order to understand the chemical speciation of small molecules in soybean dreg, they were was extracted with petroleum ether using a Soxhlet extractor. The petroleum ether extract was analyzed by Fourier transform infrared （FT-IR） spectroscopy and gas chromatography/mass spectrometry （GC/MS）. The raw bean dreg and the residue from the extraction were also analyzed by FT-IR spectroscopy. A pale yellow oil was obtained from the petroleum ether extract after 5-hour continuous extraction, with an extraction yield of 10.7%. FT-IR band shapes were similar for the raw material and the residue from extraction, but with different absorption intensities, while obvious differences in the FT-IR spectra were found between the petroleum ether extract and raw bean dreg/the residue fi＇om the extraction. In total, 35 compounds were identified from the petroleum ether extract, which can be classified into seven groups： alkanes, alkenes, arenes, aldehydes, acids, esters, and other species. Among them, the esters were the most abundant compounds, with a relative content of 61.79%. This study was important for the basic theory on developing the value-added utilization of soybean dreg,