高迁移率族蛋白B1和肿瘤坏死因子-α在强直性脊柱炎中的相关性研究

Research on the correlation between HMGB1 and TNF-α in serum of patients with ankylosing spondylitis

目的探讨高迁移率族蛋白B1(HMGB1)与肿瘤坏死因子-α(TNF-α)在强直性脊柱炎(AS)活动期患者血清中的表达及其相关性,为AS病情活动的评估提供新的血清学指标。方法应用酶联免疫吸附试验(ELISA)检测AS患者血清中HMGB1和TNF-α水平,并进行相关性分析。结果 40例AS患者血清中HMGB1与TNF-α表达水平的相关性分析显示,HMGB1与TNF-α呈正相关,并具有显著相关性(r=0.37,P=0.00)。HMGB1和TNF-α分别与Bath强直性脊柱炎病情活动指数(BASDAI)、活动期指标红细胞沉降率(ESR)、C-反应蛋白(CRP)进行相关性分析显示,HMGB1与BASDAI、ESR、CPR的升高具有一致性(rBASDAI=0.34,P=0.04;rESR=0.40,P=0.01;rCRP=0.66,P=0.00)。TNF-α与BASDAI、ESR、CRP的升高也具有一致性(rBASDAI=0.38,P=0.02;rESR=0.58,P=0.00;rCRP=0.76,P=0.00)。结论 HMGB1作为促炎因子在AS活动期与TNF-α具有相关性,抗HMGB1治疗可能为AS的治疗提供新的途径。

[Objective] To investigate the correlation between HMGB1 and TNF-α in serum of AS group. [Methods] Enzyme-linked immunosorbent assay （ELISA） was used to measure the serum level of HMGB1 and TNF-α in AS group and healthy controls. [Results] There was statistically significant positive correlation between HMGB1 and TNF-α in serum of AS patients （r=0.368, P=0.00）. Through analyzed the serum levels of HMGB1, TNF-α and bath ankylosing spondylitis disease activity index （BASDAI）, activity indexes of AS （ESR, CRP） in AS group by pearson correlation analysis, there were statistically significant positive correlations between HMGB1 levels and BASDAI, ESR, CRP （rBASDAI= 0.342, P= 0.041; rESR= 0.402, PESR= 0.012, rPLT = 0.286, PPLT = 0.028; rCRP = 0.658, PCRP = 0.00）. And TNF-α correlated with BASDAI, ESR, CRP （rBASDAI = 0.377, P= 0.016; rESR = 0.580, PESR = 0.00; rCRP = 0.761, PCRP = 0.00） positively too. [Conclusion] HMGB1 and TNF-α was correlated with AS in activity period of the disease.Anti-HMGB1 therapy may provide a new way for the treatment to AS.