芦荟提取液及其降解液制备凝胶的抑菌性研究

The Antibacterial Effects of the Gel Prepared by Aloe Extract and Degradation Solution

以芦荟提取液及其不同条件降解液制备的芦荟凝胶为研究对象,通过单因素试验和正交试验,研究不同添加剂对芦荟凝胶抑菌效果的影响,优化抑菌芦荟凝胶的添加剂配方。结果表明,制备的芦荟凝胶对大肠杆菌和金黄色葡萄球菌均具较好的抑菌效果,且不同降解液制备的凝胶抑菌性不同,说明降解条件影响了芦荟液中的抑菌活性物质,故表现出不同的抑菌效应。本试验优化结果表明,抑制大肠杆菌的芦荟凝胶添加剂最佳配方为:尿囊素浓度3.0 mg/m L,10%(V/V)三乙醇胺水溶液0.010 m L/m L,20%(W/V)Na OH水溶液0.015 m L/m L,卡松添加量为0.8μL/m L;抑制金黄色葡萄球菌的芦荟凝胶添加剂最佳配方为:尿囊素浓度3.6 mg/m L,10%(V/V)三乙醇胺水溶液0.010 m L/m L,20%(W/V)Na OH水溶液0.015 m L/m L,卡松添加量为1.0μL/m L。

Taking the aloe gel with aloe extract and its degradation under different degradation conditions as the research objects,the effects of different additives on the antibacterial effect of the aloe gel were studied and the additive formulation of antibacterial aloe gel was optimized by single factor and orthogonal tests.The results showed that,the prepared aloe gel had good antibacterial effects on Escherichia coli and Staphylococcus aureus,and the antibacterial effect of aloe gel which prepared by degradation liquid was different.The degradation conditions affected the antimicrobial substances in aloe gel,so they showed different inhibitory effects.The optimization result showed that,the best additive formula of inhibition on E.coli was as follows：allantoin concentration 3.0 mg/m L,10%（V/V） triethanolamine aqueous solution 0.010 m L/m L,20%（W/V） Na OH aqueous solution 0.015 m L/m L and kathon 0.8 L/m L;the best additive formula of inhibition on S.aureus was as follows：allantoin concentration 3.6 mg/m L,10%（V/V） triethanolamine aqueous solution 0.010 m L/m L,20%（W/V）Na OH aqueous solution 0.015 m L/m L and kathon1.0 μL/m L.