碲化镉量子点对小鼠肾脏的氧化损伤作用

Oxidative damage effect induced by CdTe Quantum Dots on kindey of mouse

目的探讨碲化镉量子点(CdTe QDs)对小鼠肾脏的氧化损伤作用。方法将40只雄性ICR小鼠随机分为5组,每组8只动物,尾静脉注射CdTe QDs溶液染毒。染毒组染毒浓度分别为0.5、5、50和500 nmol/ml,每只动物注射0.2 ml;对照组注射等体积的生理盐水,染毒24 h后小鼠脱臼处死。采用生化方法检测肾脏组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,免疫组化法观察肾脏细胞8-羟基脱氧鸟嘌呤(8-OHdG)表达水平、TUNEL法检测肾细胞凋亡。结果 500 nmol/ml CdTe QDs染毒组MDA含量显著增加,与对照组比较差异有统计学意义(P<0.01);5、50和500 nmol/ml CdTe QDs染毒组SOD和CAT活性与对照组比较显著降低(P<0.01);免疫组化法观察0.5、5、50、500nmol/ml CdTe QDs染毒组肾细胞核内均可见棕褐色的8-OHd G阳性颗粒表达,TUNEL法检测可见50、500 nmol/ml CdTe QDs染毒组有凋亡细胞阳性颗粒表达。结论本实验条件下CdTe QDs可对小鼠肾脏组织产生一定程度的氧化损伤作用,并有一定的剂量-效应关系。

Objective To investigate the oxidative damage effects of cadmium tungstate Quantum Dots （CdTe QDs） in kindey of mouse. Methods Forty ICR mice were randomly divided into 5 groups ： control group （ normal saline） ; and four CdTe QDS groups （ exposed to 0, 0. 5, 5, 50 and 500 nmol/ml CdTe QDS solution 0. 2 ml by intravenous injection, respectively）. All mice were decapitated 24 h later after the injection, then the concentration of MDA, the activities of SOD, CAT and the expression of 8-OHdG in kidney tissue homogenates were examined by biochemistry or immunohistochemistry techniques, and hepatocellular apoptosis was measured too with TUNEL at the same time. Results The results showed that the MDA concentration of kindey tissue in 500 nmol/ml CdTe QDs group was significantly higher than that of control group （ P 〈 0. 01 ） ; the activities of SOD and CAT of kidney in 5, 50, 500 nmol/ml CdTe QDs groups were significantly lower than those of control group （P 〈 0.01 ） ; the positive staining of 8-OHdG appeared in 0. 5, 5, 50 and 500 nmol/ml CdTe QDs groups and the apoptosis of kindey cells was observed in 50 and 500 nmol/ml CdTe QDs group by TUNEL technique. Conclusion It was suggested that CdTe QDs has some oxidative damage effect on mice kindey and shows some dose-response relationship.