SPARC在肝癌细胞中负性调控糖酵解作用的研究

Research on the Negative Regulatory Role of SPARC in Glycolysis in Hepatocarcinoma Cells

目的:探究肝癌细胞中的分泌性富含半胱氨酸的酸性蛋白(secreted protein acidic and rich in cysteine,SPARC)的表达与糖酵解作用的关系。方法:用SPARC质粒和SPARC siRNA分别转染人肝癌细胞株HepG2,转染后采用比色法检测细胞的葡萄糖摄取量以及乳酸生成量的变化。结果:转染SPARC质粒后的HepG2细胞的葡萄糖摄取量和乳酸生成量较对照组下降(P<0.05);转染SPARC siRNA后的HepG2细胞的葡萄糖摄取量和乳酸生成量较对照组增加(P<0.05)。结论:在肝癌细胞中,SPARC对糖酵解过程具有负性调控作用。

Objective：To investigate the relationship between the expression of secreted protein acidic and rich in cysteine （SPARC）and the glycolysis in hepatocarcinoma cells.Methods：HepG2 hepatocarcinoma cells were transfected with SPARC plasmid and SPARC siRNA,respectively.The change of glucose uptake and lactate generation after transfection was assayed by colourimetry method.Results：The glucose uptake and lactate generation decreased in HepG2 transfected with SPARC plas-mid,compared with the control group （P 〈0.05）.However,the glucose uptake and lactate generation increased in HepG2 transfected with siRNA SPARC,compared with the control group（P 〈0.05）.Conclusions：SPARC negatively regulates gly-colysis in hepatocarcinoma cells.