苯暴露工人尿中8-OHdG、tt-MA和S-PMA含量的测定

Determination of 8-OHd G,tt-MA and S-PMA contents in urine of benzene-exposed workers

目的测定苯暴露工人尿中苯巯基尿酸（S-PMA）、反-反式粘糠酸（tt-MA）和8-羟基脱氧鸟苷（8-OHd G）的含量,找出影响其变化的因素,探讨其作为苯暴露生物监测标志物的适用性。方法对苯暴露工人的作业环境进行监测,通过统一的问卷调查收集不同苯浓度暴露工人的姓名、年龄、饮酒、吸烟、职业史等一般信息,采集当日班前与班末尿样,用高效液相色谱-串联质谱法（HPCL-MS/MS）测定尿中8-OHd G、tt-MA和S-PMA含量,计算三者肌酐校正值,并对结果进行统计分析。结果苯接触工人尿中S-PMA检出率为39.2%,浓度范围在0.3-1 572.6μg/g Cr。尿中反tt-MA检出率为98.2%,检出的浓度范围为3-12 055.4μg/g Cr。;尿中8-OHd G检出率为97.2%,检出浓度范围为0.3-35.3μg/g Cr。不同性别、不同吸烟、饮酒量的工人尿中3种生物标志物的含量变化差异无统计学意义。不同苯暴露水平的工人,班前班末尿中8-OHd G、tt-MA和S-PMA的含量变化差异有统计学意义P〈0.01。结论 HPCL-MS/MS可同时检测尿中8-OHd G、tt-MA和S-PMA的含量,苯暴露工人尿中8-OHd G、tt-MA比S-PMA检出率高,尿中tt-MA的含量测定作为苯暴露生物监测指标,其效果优于8-OHd G和S-PMA。

[Objective]To determine the contents of S-phenylmercapturic acid（ S-PMA）,trans,trans-muconic acid（ tt-MA） and8-hydroxydeoxyguanosine（ 8-OHd G） in urine of benzene-exposed workers,identify possible factors affecting their change,and explore its applicability as biological monitoring marker of benzene exposure. [Methods]The working environment of benzene-exposed workers was monitored,and the questionnaire investigation was used to collect the general information（ including name,age,drinking,smoking and occupational history） of workers exposed to different benzene concentration. By using HPCL-MS / MS,the contents of S-PMA,tt-MA and 8-OHd G were detected in urine samples before and after duty,to calculate the correction by creatinine,and the results were analyzed statistically. [Results]The detection rate of S-PMA in urine of benzene-exposed workers was 39. 2%,with the concentration range of 0. 3-1 572. 6 μg / g Cr. The detection rate of tt-MA was 98. 2%,with the concentration range of 3-12 055. 4μg /g Cr. The detection rate of 8-OHd G was 97. 2%,with the concentration range of 0. 3-35. 3 μg /g Cr. There were no significant differences in contents of three biomarkers among workers in different sex,smoking,drinking groups. Among workers exposed to different benzene concentration,there were significant differences in contents of S-PMA,tt-MA and 8-OHd G between urine samples before and after duty（ P 〈0. 01）. [Conclusion]HPCL-MS / MS can simultaneously determine the contents of S-PMA,tt-MA and 8-OHd G in urine of benzene-exposed workers. The detection rates of 8-OHd G and tt-MA are higher than that of S-PMA in urine of benzene-exposed workers. The content of tt-MA in urine is a better biological monitoring marker of benzene exposure than 8-OHd G and S-PMA.