HPLC法测定绞股蓝中5种皂苷成分的含量

Quantification of five saponins in Gynostemma Pentaphyllum(Thunb.)Makino by HPLC

目的:HPLC法比较不同地区绞股蓝中人参皂苷Rb1、Rb3、Rd和绞股蓝皂苷XLIX、XVII的含量。方法:采用Shimpack C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈和水,流速为0.8m L·min-1,检测波长为203nm,柱温为25℃。结果:采自陕西沣峪口的绞股蓝样品中人参皂苷Rb1、Rb3和Rd的含量最高,约为1.625%,1.776%,0.892%;云南普洱样品所含的绞股蓝皂苷XLIX最高,约为1.75%;重庆北碚区-1的样品中XVII的含量最高,约为1.063%。结论:各地区绞股蓝样品中皂苷含量差异很大。

Objective：To compare the content of Saponins in Gynostemma pentaphyllum（Thunb.） Makino from different regions. Methods ：The HPLC separation was carried out with a C18column（4.6mm ×250mm,5μm）using acetonitrile and water as mobile phase at a flow rate of 0.8m L·min-1. The detection wavelength was203 nm and the column temperature was maintained at 25℃. Results：The material collected from Fengyukou in Shaanxi had the highest content of ginsenoside Rb1,Rb3 and Rd,about 1.625%,1.776%,0.892% respectively.The material from Pu＇er in Yunnan contained the highest Gyp（Gypenoside） XLIX,approximately 1.75%. The material from Chongqing Beibei-1 had the highest content of Gyp XVII,about 1.063%. Conclusion：There was a significant difference in the content of Saponins in Gynostemma pentaphyllum from the different areas.