实时荧光聚合酶链式反应法检测食品中狗源性成分

Detection for dog-derived components in foods by real-time polymerase chain reaction

根据狗线粒体NADH氧化还原酶亚基2基因(ND2)中的序列设计了狗特异性引物和Taqman探针,建立了食品中狗源性成分的实时荧光聚合酶链式(Real-time PCR)检测方法。实验表明,该方法可以很好地区分狗与其他常见的14个物种,引物、Taqman探针特异性良好,检测灵敏度可达100fg,适用于食品中狗源性成分的快速鉴定。

A rapid and highly species-specific real-time polymerase chain reaction（ PCR） assay had been developed for the detection of dog-derived components in meat and meat mixtures. Primers and Taqman probe were designed on the mitochondrial ND2,and the performance of the method was tested. Results showed that no cross-reaction was observed between the dog and non-target species,and this method revealed a high sensitivity and could detect 100 fg of dog template DNA. In conclusion,this real-time PCR assay could be a rapid and straightforward method for the accurate identification of dog-derived components in food.