灵芝深层发酵产物抗氧化活性物质与抗氧化能力分析

Analysis of antioxidant substances and antioxidant capacity of submerged fermentation product of Ganoderma Lucidum

以不同灵芝菌株液体发酵产物作为研究材料,测定抗氧化活性物质含量,并利用多种抗氧化测定方法,分析不同菌株的体外抗氧化能力,评价活性物质含量间、抗氧化方法间及抗氧化方法与含量间的相关性。结果表明,供试菌株活性物质含量间的差异较大,紫芝13、德昌1号黄酮含量最高,而京大灵芝多糖、三萜、多酚含量显著高于其余菌株(p<0.05),且四种活性物质含量间相关性不大;不同灵芝菌株抗氧化活性不同,其中京大灵芝具有较高的还原力,白灵芝、金地灵芝、有柄树舌2949 DPPH自由基清除能力较高,通江灵芝、紫芝101羟自由基清除能力较强,韩芝有较高的超阴离子自由基清除能力,且不同方法间存在较大差异,显著性不明显;四种活性物质含量与各抗氧化测定方法间相关系数低,表明灵芝的抗氧化能力贡献并不完全来自这四类物质。

The objectives of this study were to analysis the differences of content of active antioxidant substances and the differences of antioxidant activity of liquid fermentation products from G.lucidum by a variety of antioxidant methods,and to further investigate the correlative relationships between contents of different active components,between results from different antioxidant assay methods and between contents of different active components and results from different antioxidant assay. The results showed that the contents of active substances on different strains had obvious difference,where the contents of flavonoid from ZZ13 and DC1 were the highest than those form others,and the contents of polysaccharide,triterpenes,polyphenol from JD were the highest（p 0.05）. The correlation among active substances was low. The antioxidant activities of different strains were different,in which JD had a high reducing power,TJ and ZZ101 had the strongest effect on hydroxyl radical scavenging,BZ,Jindi and SS2949 had the strongest DPPH scavenging,and HZ had the highest superoxide radical scavenging effect. And there were significant differences among different methods,and significance was not obvious. The correlation coefficient between contents of four active substances and antioxidant was low,which indicated that the four active substances did not important contribute to antioxidant capacity from G.lucidum.