葡萄枝条提取物的抑菌活性及其在红地球葡萄防腐保鲜上的应用研究

Anti-microbial activity of grape cane extracts and its application on storage of Red Globe grape

采用微量肉汤稀释法研究了葡萄枝条提取物对供试3种真菌和5种细菌的最小抑菌浓度(MIC),结果表明葡萄枝条提取物对真菌和细菌均有一定的抑菌活性;为进一步明确葡萄枝条提取物在葡萄防腐保鲜中的应用,以红地球葡萄为试材,分别以葡萄枝条提取物、壳聚糖及葡萄枝条提取物与壳聚糖复合溶液作为保鲜剂,研究了其对葡萄防腐保鲜的作用效果,结果表明葡萄枝条提取物与壳聚糖复合涂膜处理能够降低葡萄的失水率、保持可溶性固形物含量和含酸量等品质指标,同时对降低果皮上真菌和细菌菌落总数作用显著(p<0.05)。

Micro broth dilution method was employed to investigate the minimal inhibitory concentration（MIC） of the grape cane extracts to 3 kinds of fungi and 5 kinds of bacteria. The results showed that the grape cane extracts had significant effect on inhibiting the activities of selected microorganisms. In order to acquire more information about the application of grape cane extracts on table grape storage,compared the preservative effect of three substrates,i.e. grape cane extracts,chitosan,and the mixture of above two substrates on table grape（Vitis vinifera L. cv. Red Globe）. It was found that preservative films smearing with grape cane extracts and chitosan efficiently prevented the clusters from serious dehydration,helped sustain the soluble solid content and titratable acidity of berries,and significantly reduced（p〈0.05） the total plate count of fungi and bacteria on the berry surface.