疏肝健脾方对CCl_4致大鼠肝纤维化的治疗作用动态观察

Dynamic Study on Curative Effect of Shuganjianpifang Against Hepatic Fibrosis Induced by CCl_4 in Rats

目的:动态观察疏肝健脾方对四氯化碳(CCl4)诱导大鼠肝纤维化的治疗作用。方法:于SD大鼠背部皮下注射50%CCl4,1 m L/kg,2次/w,连续12 w以制作肝纤维化模型。于造模第1周起,给药组分别灌胃给予疏肝健脾方和秋水仙碱(阳性对照),1次/d,连续16 w,动态观察造模后4、8、12、16 w 4个不同时间节点各组动物肝脏组织病理学变化,血清ALT、AST、GSH及肝组织SOD、MDA水平的动态变化。结果:与正常对照组比较,模型组大鼠在第4、8、12、16周血清ALT、AST及肝组织MDA水平显著升高,及肝组织SOD及血清GSH水平显著降低;与模型组比较,在8、12、16周,疏肝健脾方组与秋水仙碱组血清ALT、AST及肝组织MDA水平显著降低,及肝组织SOD及血清GSH水平显著升高。HE染色显示,疏肝健脾方组各时间段肝纤维化程度均较模型组减轻。结论:疏肝健脾方在不同时间节点对CCl4诱导的大鼠肝纤维化均具有很好的保护作用。

Objective： To observe the dynamic effects of Shuganjianpifang（SGJPF） in preventing rat hepatic fibrosis induced by CCl4. Methods： The model rat was induced by subcutaneous injection of 50% CCl4 solution in olive oil（0. 1 m L /100 g）,twice a week for 12 weeks. The treatment groups were given by lavage with SGJPF and Colchicine respectively once a day for 16 weeks. After 4,8,12 and 16 weeks,six rats were randomly sacrificed in each group. Alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,and glutathione（GSH） in serum as well as superoxide dismutase（SOD） and malondialdehyde（MDA） in hapatic tissues were examined by colorimetric methods dynamically. Meanwhile,the histopathologic changes of hepatic tissues were observed by the HE stained method. Results： Compared with normal group,the levels of ALT and AST in serum and MDA in hepatic tissues were significantly elevated,the levels of SOD in hepatic tissues and GSH in serum were significantly decreased in model group after 4,8,12 and 16 weeks. Compared with model group,the levels of ALT and AST in serum and MDA in hepatic tissues were significantly reduced,the levels of SOD in hepatic tissues and GSH in serum were increased significantly after 8,12 and 16 weeks in SGJPF group and Colchicine group. Histopathologic examination results also showed that SGJPF reduced the degree of liver fibrosis after 4,8,12 and 16 weeks. Conclusion： Shuganjianpifang can obviously inhibit the formation of liver fibrosis at different phases in rats.