血根碱对小鼠S_(180)皮下移植瘤的抑制作用

Inhibition of Sanguinarine on S_(180) Subcutaneously Implanted Tumors in Mice

目的:探讨血根碱对S180荷瘤小鼠的抑瘤作用及对血管生成的影响。方法:建立小鼠S180皮下移植瘤模型,随机分为模型组、环磷酰胺(CTX 20 mg/kg)组、血根碱(10、20、40 mg/kg)组、联合组(血根碱10 mg/kg+CTX20 mg/kg),连续给药10 d后处死小鼠,检测移植瘤质量及体积并计算抑瘤率;HE染色观察肿瘤细胞的形态学特征;免疫组化法检测药物对微血管密度(MVD)、血管内皮生长因子(VEGF)表达的影响。结果:与模型组比较,CTX组、血根碱(20、40 mg/kg)组、联合用药组肿瘤生长显著受到抑制;肿瘤组织HE染色显示,与模型组比较给药组中肿瘤细胞异型性降低,病理性核分裂减少,可见散在及融合成片状的坏死灶;免疫组化显示CTX、血根碱(20、40mg/kg)及联合用药均能显著减少MVD、VEGF的表达,联合用药时抑瘤效果更显著(P<0.01,P<0.05)。结论:血根碱可通过下调S180移植瘤组织中MVD、VEGF的表达,从而抑制肿瘤血管的生成发挥抗肿瘤作用。

Objective： To investigate the inhibition of sanguinarine on S180 sarcoma in mice and the effect of angiogenesis. Methods：S180subcutaneous implanted tumor model mice were randomly divided into six groups： control group,cyclophosphamide（CTX） group,sanguinarine（10,20 and 40 mg/kg） groups and combination group. The mice were sacrificed on the 10 thday to measure the tumor weight and volumes,and caculate the tumor growth inhibition. Histopathology was performed,while immunohistochemistry was applied for assessment of MVD（microvascular density） and the expression of VEGF（vascular endothelial growth factor）. Results： The growth of tumors were significantly inhibited in the treatment groups（CTX group,sanguinarine 20 and 40 mg/kg groups,and combination group）.HE staining showed tumor cell atypia and pathologic micosis were lower than that of the control group. Scattered and fusion of the slice necrosis foci were observed in the treatment groups. CTX,sanguinarine（20 and 40 mg/kg） and combination significantly reduced the expression of MVD and VEGF compared with the control group（P〈0. 01,P〈0. 05）. Conclusion： Sanguinarine can effectively inhibit the growth of S180 implanted tumors via reducing MVD and the expression of VEGF,which is associated with its anti-angiogenesis.