摘要
本实验用超临界CO2流体萃取的方法从生姜中提取姜辣素,通过三种不同的自由基体系和小鼠B16黑色素瘤细胞模型研究了生姜姜辣素的抗氧化活性。超临界CO2流体萃取制得的生姜油中姜辣素的含量为263.42mg/g。体外抗氧化实验结果表明,在一定浓度范围内,与维生素C相比,生姜姜辣素同样具有较强的抗氧化能力。同时,细胞实验说明,生姜姜辣素能够抑制小鼠B16黑色素瘤细胞的生长。从形态学观察,随着姜辣素浓度的升高,对小鼠B16黑色素瘤细胞的生长抑制情况越明显;MTT实验中,随着姜辣素浓度的升高和时间的延长,其对小鼠B16黑色素细胞的抑制作用越大;流式细胞仪检测浓度为50μg/m L的生姜姜辣素可使小鼠B16黑色素瘤细胞产生凋亡。
The supercritical CO2 fluid extraction technology （SFE） was used to extract gingerol from ginger. This experiment determined the antioxidant activity of gingerol through ihree different kinds of free radical systems and mouse B16 melanoma cell model in vitro. The extraction yield of gingerol in ginger oil could be up to 263.42mg/g through SFE. The results indicated that, compared with Vc, gingerol also had a very strong antioxidant activity in a certain range of concentration by different antioxidants tests, including Superoxide, DPPH and hydroxyl radical scavenging. The experiment showed that gingerol inhibited the growth of mouse B16 melanoma cells and induced apoptosis by MTT assay. With the increase of gingerol content and time, inhibition of growth of B16 melanoma cells was enhanced. Meanwhile, flow cytometry analysis showed that gingerol with the concentration of 50μg/mL could lead to the apoptosis of mouse B16 melanoma cells.
出处
《中国食品添加剂》
CAS
北大核心
2014年第8期96-101,共6页
China Food Additives
