固相萃取-梯度高效液相色谱法同时测定婴幼儿食品中维生素A、D、E的含量

Determination of vitamin A D and E in infant food by gradient elution-solid phase extraction-high performance liquid chromatography

目的：建立固相萃取一高效液相色谱法测定婴幼儿食品中的VA、VD、VE含量的方法。方法：在已有研究基础上对样品前处理方法进行了优化和改进，皂化后利用正己烷：甲基叔丁基醚（v：v=1：1）进行提取，PLS小柱萃取分离，50％丙酮正己烷洗脱，甲醇定容后高效液相色谱仪梯度洗脱检测。结果VA、VD、VE在0．1～10μg／mL浓度范围内相关系数均为0．999以上，回收率均在90％以上，相对标准偏差小于4％。结论：方法快速、灵敏、准确，前处理简单易行，可用于婴幼儿食品中VA、VD、VE的同时测定。

Object： A solid phase extraction followed by high performance liquid chromatographic method was developed for the determination of vitamin A, D and E in infant food. Method： The research optimized and improved the sample pretreatment on the basis of existing study. N - hexane and methyl tert - butyl ether（ v ： v = 1 ： 1 ） were used to extract active ingredients after saponification, PLS solid phase extraction column was used and 50% acetone and n - hexane was eluted solution. The residue was fihrated with membrane and reserved for analysis after dissolved in methanol prior to analysis on a high performance liquid chromatography equipped with a UV detector by gradient elution. Results ： The correlation coefficients of the method were all above 0. 999. The average of recoveries of target drugs were better than 85% with relative standard deviations（RSDs） less than 4%. Conclusion： Because of the advantages of good linearity, low limit of detection, high precision and rapidity, this method is suitable for the determination of vitamin A, D and E in infant food.