基于靛红双席夫碱-氧化石墨烯荧光传感器快速检测柠檬黄含量的应用研究

Study on rapid detection of lemon yellow by fluorescence sensor using Isatin Double Schiff graphite oxide

对三种靛红双席夫碱的荧光性质进行了深入的研究，选出荧光强度较高的靛红缩对苯二胺双席夫碱（L4）作为产生荧光的化合物，利用席夫碱L4和柠檬黄在氧化石墨表面吸附能力不同，建立了席夫碱-氧化石墨烯荧光传感器测定了柠檬黄含量的新方法，柠檬黄浓度在8．58ng·L-1-327ng·L-1范围内，其浓度与荧光强度值具有良好的线性关系，回归方程为F=0．4454-2．9562，R=0．991，检出限为0．34ng·L-1，该方法已应用于饮料中柠檬黄含量的检测。

The fluorescence properties of three Isatin Double Schiff base was studied, the isatin shrinkage para - phen- ylene diamine Schiff base （IA） with higher fluorescence intensity was selected as fluorescent compounds. Schiff base IA and lemon yellow on graphite oxide surface had different adsorption abilities. Therefore, a new detecting method of lemon yellow was established. At the range of 8. 58ng . L-1 -327ng . L-1 lemon yellow concentration and fluorescence intensity value have a good linear relationship and the regression equation is Ip F = 0. 4454C - 2. 9562 with correlation index of 0. 991, and the detection limit is 0. 34ng . L-1. The method was applied in lemon yellow content detection in drinks.