食管鳞状细胞癌组织中FOXO3a、EGFR和CyclinD1蛋白的表达

Expressions of FOXO3a,EGFR and CyclinD1 in esophageal squamous cell carcinoma tissue

目的:检测食管鳞状细胞癌(ESCC)组织中转录因子FOXO3a、表皮生长因子受体(EGFR)、细胞周期蛋白D1(CyclinD1)的表达。方法:用免疫组化SP法检测87例ESCC及其远端正常食管黏膜组织中FOXO3a、EGFR、CyclinD1蛋白的表达。结果:FOXO3a蛋白在ESCC组织中的阳性表达率为13.8%,低于正常食管黏膜组织(70.1%;χ2=49.000,P<0.001),FOXO3a蛋白的表达与ESCC的组织学分级有关(P<0.05);EGFR蛋白在ESCC组织中的阳性表达率为64.4%,高于正常食管黏膜组织(10.3%;χ2=45.082,P<0.001),EGFR蛋白的表达与ESCC的组织学分级、浸润深度、淋巴结转移和TNM分期有关(P<0.05);CyclinD1蛋白在ESCC组织中的阳性表达率为72.4%,高于正常食管黏膜组织(14.9%;χ2=48.077,P<0.001),CyclinD1蛋白的表达与ESCC的浸润深度及淋巴结转移有关(P<0.05)。ESCC组织中FOXO3a与EGFR、CyclinD1表达均呈负关联(rp=-0.474和-0.497,P<0.05),EGFR与CyclinD1的表达呈正关联(rp=0.556,P<0.001)。结论:FOXO3a、EGFR、CyclinD1可能在ESCC的发生发展中起重要作用。

Aim：To explore the expressions and clinical significance of the transcription factor FOXO 3a, epidermal growth factor receptor（EGFR）,and cyclinD1 in esophageal squamous cell carcinoma （ESCC）tissue.Methods：Immunohis-tochemical SP staining was used to detect the expressions of FOXO 3a,EGFR,and CyclinD1 in 87 cases of ESCC and their corresponding distal normal esophageal mucosa .Results：The positive expression rate of FOXO3a in ESCC tissue was 13.8%,lower than that in normal tissue （70.1%;χ^2 =49.000,P〈0.001）,and the expression of FOXO3a was related with histological grade of ESCC（P〈0.05）.The positive expression rate of EGFR in ESCC tissue was 64.4%,higher than that in normal tissue（10.3%;χ^2 =45.082,P〈0.001）,and the expression of EGFR was related with histological grade ,depth of invasion,lymph node metastasis and TNM staging of ESCC （P〈0.05）.The positive expression rate of CyclinD1 in ESCC tissue was 72.4%,higher than that in normal tissue （14.9%;χ^2 =48.077,P〈0.001）,and the expression of CyclinD1 was related with depth of invasion and lymph node metastasis （P〈0.05）.The expression of FOXO3a was negatively associated with EGFR and CyclinD1（r p =-0.474 and -0.497,P〈0.05）,and the expression of EGFR was positively associated with that of CyclinD1 in ESCC tissue（rp =0.556,P〈0.001）.Conclusion：FOXO3a,EGFR,and CyclinD1 may play im-portant roles in the occurrence and development of ESCC .