原发性肺癌患者外周血中microRNA-31的表达及意义

Expression and significance of microRNA-31 in peripheral blood of primary lung cancer patients

目的：探讨microRNA-31（ miRNA-31）在原发性肺癌患者外周血中的表达情况及其临床意义。方法：采用实时荧光定量PCR技术检测100例原发性肺癌患者和100例正常人外周血miRNA-31表达水平。绘制ROC曲线，评价外周血miRNA-31表达水平对原发性肺癌的诊断价值。以肺癌患者外周血miRNA-31表达水平的平均值为界值，将100例肺癌患者分为外周血miRNA-31低表达组和高表达组，采用Kaplan-Meier法对两组绘制生存曲线，评价外周血miRNA-31表达水平对原发性肺癌预后判断的价值。结果：肺癌患者外周血miRNA-31表达水平为（1．93±0．88），与正常人的（0．67±0．31）比较，明显升高（t＝13．505，P＜0．001）。根据外周血miRNA-31表达水平绘制ROC曲线，曲线下面积为0．775（95％CI＝0．681～0．868），临界值取1．126时，用外周血miRNA-31表达水平诊断原发性肺癌的灵敏度和特异度分别为0．778和0．736。肺癌患者中外周血miRNA-31低表达44例，高表达56例，高表达和低表达患者的生存曲线差异有统计学意义（χ^2＝5．710， P＝0．017），低表达组中位生存期为37．32个月，长于高表达组的27．11个月。结论：miRNA-31可作为原发性肺癌诊断和预后判断的分子标志物。

Aim：To investigate the expression and clinical significance of microRNA-31（miRNA-31）in peripheral blood of primary lung cancer patients .Methods：Real-time PCR method was used to examine miRNA-31 in peripheral blood of 100 primary lung cancer patients and 100 healthy persons ,respectively .ROC curve were drawn to evaluate the diagnosis value of miRNA-31 expression in peripheral blood for primary lung cancer .Based on the average of miRNA-31 expression in peripheral blood,the 100 lung cancer patients were divided into miRNA-31 low expression group and high expression group ,Kaplan-Meier survival curves of the 2 groups were drawn .Results：miRNA-31 expression in peripheral blood of the primary lung cancer patients was （1.93 ±0.88）,higher than that （0.67 ±0.31） of the healthy persons（t=13.505,P〈0.001）.The area under the ROC curve according to the miRNA-31 expression in peripheral blood was 0.775（95%CI =0.681-0.868）,when the critical value was 1.126,the sensitivity for primary lung cancer diagnosis and specific degrees were 0.778 and 0.736,re-spectively.The difference in the survival curve between miRNA-31 low expression group（44 cases） and high expression group （56 cases） was statistically significant （χ^2 =5.710,P=0.017）,the median survival period of the low expression group was 37.32 months,longer than 27.11 months of the high expression group .Conclusion： MiRNA-31 expression in peripheral blood may be a molecular marker for the diagnosis and prognosis evaluation of primary lung cancer .