坐骨神经损伤中HMGB1对施万细胞迁移作用的研究

Study of the HMGB1 effects on migration of Schwann cells following sciatic nerve injury

目的：研究周围神经损伤后高迁移率族蛋白B1（high mobility group protein B1,HMGB1）在损伤神经远侧端的细胞定位及表达变化,探讨HMGB1对施万细胞（Schwann cells,SCs）迁移特性的影响,阐明HMGB1功能作用的细胞学基础。方法：建立Sprague-Dawley（SD）大鼠坐骨神经损伤模型,采用免疫荧光组织双标化学染色观察神经损伤后HMGB1的定位和表达情况,新生1-3 d SD大鼠取材体外培养SCs并进行纯化,利用Transwell小室检测HMGB1对SCs迁移的影响。结果：大鼠坐骨神经中HMGB1和S100共定位,主要定位于细胞核内,损伤后远侧端HMGB1表达明显升高;体外培养的SCs经过纯化后排列整齐、胞体透亮,纯度可达95%以上,可用于后续体外试验;HMGB1蛋白能够促进SCs迁移。结论：HMGB1参与损伤坐骨神经远侧端SCs的功能调节。

Objective： To investigate the localization and expression changes of high mobility group protein B1（HMGB1） following sciatic nerve injury,and also to test the effect of HMGB1 on migration of Schwann cells（SCs） in attempt to clarify the functional basis of the cells. Methods： With the establishment of rat sciatic nerve injury model,immunohistochemistry method was used to study the localization and the expression changes of HMGB1. SCs were also isolated from newborn rat pups（within postnatal 1-3 d） and purified. Transwell was used to test the effect of HMGB1 on migration of SCs. Results： Immunohistochemistry demonstrated that HMGB1 colocalized with S100 positive cells in the distal segment with most of them colocalized in nucleus,the positive staining increased after sciatic nerve injury. The purified SCs were in regular morphology. Statistical data showed that purification of the cell reached up to 95%. Extracellular HMGB1 was able to promote the migration of SCs.Conclusion： HMGB1 is involved in the functional regulation of SCs after sciatic nerve injury.