探索全氟辛烷磺酸对HAPI小胶质细胞炎症反应的影响

Perfluorooctane sulfonate induced inflammatory response by oxidative stress in HAPI microglia

目的:探讨全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)对HAPI小胶质细胞的毒性反应及作用机制。方法:用20 nmol/L PFOS处理HAPI小胶质细胞6、12 h后;采用细胞免疫荧光检测活性氧簇(reactive oxygen species,ROS)的表达;酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)的分泌量。结果:细胞免疫荧光显示HAPI小胶质细胞在PFOS处理后,ROS的表达显著增加(P<0.05);ELISA检测显示TNF-α的分泌量增加并达到(81.00±2.38)pg/m L,而对照组TNF-α为(22.00±1.63)pg/m L。结论:PFOS可以引起HAPI小胶质细胞产生氧化应激并分泌TNF-α。

Objective： To explore the toxic effects and mechanism of perfluorooctane sulfonate（PFOS） on HAPI microglia.Methods： The HAPI cells were treatd with 20 nmol/L PFOS for 6 and 12 h. The reactive oxygen species（ROS） and tumor necrosis factor-alpha（TNF-α） were quantified by immunocytochemistry and enzyme-linked immunosorbent assay（ELISA） respectively. Results： After PFOS treatment,immunocytochemistry staining suggested that ROS were obviously elevated in PFOS-exposed HAPI cells. Similarly,ELISA showed that treatment with PFOS led to 4-fold increase of TNF-α secretion[（81.00±2.38） pg/m L] compared with control groups [（22.00±1.63） pg/m L]. Conclusion： PFOS induced oxidative stress and subsequent secretion of proinflammatory factor TNF-α in HAPI microglia.