叉头翼状螺旋转录因子3及白细胞介素17在结肠癌中的表达及临床意义

Forkhead or winged helix transcription 3 and interleukin-17 expression in colon cancer and clinical significance

目的 通过观察结肠癌患者肿瘤组织及癌旁组织中叉头翼状螺旋转录因子3（Foxp3）表达情况,以及检测结肠癌患者和健康人血清白细胞介素17 （IL-17）的水平,探讨调节性T细胞（Treg细胞）和Th17细胞在结肠癌发生和发展过程中的变化.方法 采用免疫组织化学SP法检测56例结肠癌组织及癌旁组织和15例正常结肠组织中Foxp3表达情况,采用酶联免疫吸附法检测其血清IL-17水平.结果 结肠癌患者血清IL-17明显高于健康对照者[（9.1 ±2.3） ng/L比（6.2±1.5）ng/L],差异有统计学意义（P=0.007）.结肠癌组织Foxp3阳性细胞数均显著多于正常对照组织和癌旁组织[（24.1±6.4）个比（2.7±1.1）个和（8.7±2.3）个],癌旁组织显著多于正常对照组织,差异均有统计学意义（P＜0.01）.TNM分期Ⅲ期结肠癌患者血清IL-17显著高于Ⅰ～Ⅱ期[（8.5±2.1） ng/L比（5.4±0.9） ng/L],结肠癌组织Foxp3显著多于Ⅰ～Ⅱ期[（25.8±6.2）个比（18.2±4.4）个],差异有统计学意义（P＜0.01）.病理分级中高分化腺癌血清IL-17与低分化腺癌[（9.4±1.1）ng/L比（8.9±1.8）ng/L]比较差异无统计学意义（P＞0.05）;中高分化结肠癌组织Foxp3显著多于低分化结肠癌组织[（26.8±5.5）个比（17.2±3.2）个],差异有统计学意义（P＜0.01）.结论 结肠癌患者血清IL-17水平和结肠癌组织Foxp3阳性表达显著高于正常组织,且随TNM分期及病理分级增高而增高,提示IL-17以及Foxp3在结肠癌的生长和转移过程中发挥重要作用.IL-17水平和Foxp3表达与局部肿瘤组织的免疫状态密切相关,联合检测有助于进一步了解肿瘤患者的免疫状态,为肿瘤的免疫治疗提供基础资料.

Objective To observe the expression of forkhead or winged helix transcription 3 （Foxp3） in colon cancer tissue and paracancerous tissue,and detect the level of serum interleukin （IL）-17 in colon cancer patients and healthy human,to explore the changes of regulatory T cell （Treg cell） and Th17 cell in the process of occurrence and development of colon cancer.Methods The expression of Foxp3 in 56 patients with colon cancer and paracancerous tissue and 15 cases with normal colon tissue was measured by immunohistochemical SP method and the level of serum IL-17 was determined by enzyme-linked immunosorbent method.Results The level of serum IL-17 in colon cancer tissue was higher than that in normal colon tissue [（9.1 ± 2.3） ng/L vs.（6.2 ± 1.5） ng/L],and there was significant difference （P =0.007）.Foxp3 positive cell number in colon cancer tissue was more than that in normal colon tissue and paracancerous tissue （24.1 ± 6.4 vs.2.7 ± 1.1 and 8.7 ± 2.3）,paracancerous tissue was more than normal colon tissue,and there was significant difference （P ＜ 0.01）.The level of serum IL-17 in colon cancer tissue with TNM Ⅲ was higher than that in TNM Ⅰ-Ⅱ [（8.5 ± 2.1） ng/L vs.（5.4 ± 0.9） ng/L],Foxp3 was more than that in TNM Ⅰ-Ⅱ （25.8 ± 6.2 vs.18.2 ± 4.4）,and there was significant difference （P＜ 0.01）.There was no significant difference in the level of serum IL-17 between middle-high differentiated adenocarcinoma and low differentiated adenocarcinoma [（9.4 ± 1.1） ng/L vs.（8.9 ± 1.8） ng/L] （P ＞ 0.05）.Foxp3 in middlehigh differentiated adenocarcinoma was more than that in low differentiated adenocarcinoma （26.8 ± 5.5 vs.17.2 ± 3.2）,and there was significant difference （P ＜ 0.01）.Conclusions Detection of IL-17 and Foxp3 can provide a new way of targeting therapy for colon cancer.IL-17 levels and Foxp3 expression are closely related to the immune status of local tumor tissues,the joint detection is benefitial to the further understanding of the patients with tumor immune state,provide basic information for tumor immunotherapy.